DNA is most commonly extracted from plant tissues using cetyltrimethylammonium bromide (CTAB), a detergent used to lyse cells and release genomic DNA from the protoplast. The Doyle and Doyle (1987) CTAB protocol publicized its efficiency and versatility across species and detailed numerous modifications to optimize extractions across plant groups.

Cetyltrimethylammonium bromide (CTAB)–based methods are widely used to isolate DNA from plant tissues, but the unique chemical composition of secondary metabolites among plant species has necessitated optimization.

In the search for an economical protocol for plant DNA extraction that we could apply uni-versally, we adopted a modification of a relatively simple high salt CTAB protocol that, by using a pre-wash with a sorbitol-containing buffer, aims to remove interfering contaminants from tissue macerates prior to cell lysis.

2023年6月2日 · We recognize eight protocol steps that are applied during CTAB extractions (Figure 1): tissue preparation, suspension, lysis, isolation, cleaning, elution, secondary cleanup, and quantification. Most protocols, however, did not include all eight steps, and often the suspension and secondary cleanup were omitted.

CTAB buffer is a cationic detergent that facilitates cell lysis and prevents secondary metabolites from interfering with DNA extraction. Following plant cell lysis, RNase A is added to digest RNA, and DNA is separated from other cellular components using phenol/chloroform extraction, which separates the sample into two distinct aqueous and ...

2022年1月1日 · Our optimized CTAB protocol has been adjusted by the addition of Sodium Dodecyl Sulfate (SDS) and proteinase K during the lysis phase. The combined effect of these reagents was capable of lysing cell walls and removing proteins efficiently.

2019年2月17日 · Plant DNA extraction needs a CTAB DNA extraction buffer that contains ingredients that can effectively remove polysaccharides and polyphenols. Let's see how it works and how to optimize the CTAB buffer for plant DNA extraction.

2025年2月28日 · CTAB (cetyltrimethylammonium bromide) buffer has been used in many protocols with some variations and is widely used for DNA extraction from diverse plant species and taxa. ... The CTAB protocol has been modified in many ways by the addition of chemical additives to the suspension or lysis buffer to reduce the phenolic compound and ...

2023年5月27日 · We recommend DNA extractions of silica gel-preserved tissues that include a shorter and cooler lysis step, which results in purer extractions compared to a longer and hotter lysis step, while preventing fragmentation and reducing time.

2023年5月27日 · Premise: The use of cetyltrimethylammonium bromide (CTAB) is an effective and inexpensive method of extracting DNA from plants. The CTAB protocol is frequently modified to...