2018年5月5日 · USP8 reduces both multiple monoubiquitination and polyubiquitination of Cx43 to prevent autophagy-mediated degradation. Consistently, knockdown of USP8 results in decreased Cx43 protein levels in cultured cells and suppresses intercellular communication, revealed by the dye transfer assay.

2023年10月30日 · After treatment with TSZ for 10 h, S454A mutant of MLKL increased the K48-linked ubiquitination of Cx43 (Fig. 3C, lane 5, K48-Ub) in contrast to MLKL (WT) (lane 4), but not K63-linked ubiquitination (Fig. 3C, lane 5, K63-Ub). These findings suggest that MLKL inhibits Cx43 ubiquitination, mainly by inhibiting K48-linked ubiquitination of Cx43.

2011年7月15日 · The tyrosine-sorting signal (YXXØ), present at amino acids 286-289 of Cx43 (connexin43), has been implicated in the internalization of the protein. In recent years, ubiquitination of Cx43 has also been proposed to regulate gap junction intercellular communication; however, the underlying mechanism and …

2011年6月28日 · Immunofluorescence, cycloheximide-chase and cell-surface-protein biotinylation experiments demonstrate that oligomerization of Cx43–Ub into hemichannels containing wild-type Cx43 or mutant Cx43Y286A is sufficient to drive the internalization of the protein.

2015年5月1日 · By confocal microscopy, we show that ischemia induces extensive co-localization of ubiquitin and Nedd4 with Cx43 localized at IDs. Moreover, by subcellular fractionation and co-immunoprecipitation assays, we demonstrate an increased interaction with Nedd4 and ubiquitination of Cx43 localized at IDs.

2012年6月1日 · As shown in Figure 6H, interaction between p62 and Cx43 significantly increased in cells expressing Cx43-Ub. Taken together, these data suggest that Nedd4-mediated ubiquitinylation of Cx43 is required to initiate the degradation of GJ Cx43 by macroautophagy.

In this study, we demonstrate that Cx43 is modified with lysine 63-linked polyubiquitin chains and that these increase the interaction between Cx43 and AMSH. We also show that AMSH is recruited to GJ plaque sites at the plasma membrane, …

2018年5月1日 · USP8 reduces both multiple monoubiquitination and polyubiquitination of Cx43 to prevent autophagy-mediated degradation. Consistently, knockdown of USP8 results in decreased Cx43 protein levels in cultured cells and suppresses intercellular communication, revealed by the dye transfer assay.

2020年3月5日 · In the present work, we demonstrate that knockdown of EHD1 impaired the internalization of Cx43, preserving gap junction-intercellular coupling in cardiomyocytes. Interaction of Cx43 with EHD1 was mediated by Eps15 and promoted by phosphorylation and ubiquitination of Cx43.

Here, we report that the ubiquitin-specific peptidase 8 (USP8) interacts with and deubiquitinates Cx43. USP8 reduces both multiple monoubiquitination and polyubiquitination of Cx43 to prevent autophagy-mediated degradation.