Cy5 is reactive, water-soluble, and has an absorbance maximum of 649 nm and an emission maximum of 670 nm. It is available as both a phosphoramidite and an NHS ester, and is used …

The 5-Cy5 modification incorporates a fluorescent Cy™ 5 dye at the 5' terminus of an oligonucleotide. 5' Cy5 modified oligonucleotides can be used in a wide array of applications including dual-labeled fluorogenic probes for real-time PCR.

Oligo d(T)12-18 is the classic primer mix used to prime synthesis of the first strand cDNA by reverse transcriptase using poly A+ mRNA as a template. Oligo dT of various sizes are synthesized individually and gel purified.

5' Cy5™ Return to Fluorophores Modifications Freedom™ DyesIDT product details for 5' Cy5™

2025年3月11日 · ReadiLink™ Cy5 Oligo and ssDNA Labelling Kit enables simple and uniform tagging of single-stranded DNA or oligos with Cy5 fluorophore. The labelling kit uses our proprietary TAQuest™ terminal deoxynucleotidyl transferase (TdT) to catalyze non-template directed nucleotide incorporation onto the 3’- end of single-stranded DNAs or oligos.

2024年10月28日 · Cy5标记的寡核苷酸（Cy5-labeled oligonucleotides，ODN）是指通过将近红外荧光染料Cy5与寡核苷酸（短链DNA或RNA分子）共价连接而成的标记物。 这类标记物广泛应用于分子生物学研究、基因表达分析和生物传感器开发。

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ReadiLink Cy5 Oligo 和 ssDNA 标记试剂盒能够使用 Cy5 荧光团对单链 DNA 或寡核苷酸进行简单而统一的标记。 标记试剂盒使用我们专有的 TAQuest 末端脱氧核苷酸转移酶 (TdT) 催化非模板定向核苷酸掺入到单链 DNA 或寡核苷酸的 3'-末端。

For each new Cy5 dye analogue prepared above, five different DNA oligonucleotide sequences were synthesized with each dye internally incorporated into the target oligo during automated synthesis.

The kit is optimized for efficient labelling and contains all the essential reagents required for efficient labelling of ssDNA or oligos. The resulting Cy5-labelled DNA probes are ideally suited for biological applications, e.g., electrophoretic mobility shift assays (EMSA), Northern and Southern blots, colony or in situ hybridizations.