1985年11月25日 · We demonstrate that the levels of cya mRNA in crp- cells are significantly higher than that in wild type cells and that the synthesis of cya RNA is dramatically reduced by …

The cya-5 nuclear mutant of Neurosopora crassa was previously shown to be deficient in cytochrome aa 3, cytochrome c oxidase activity, and the immunologically detectable COXI …

2022年8月18日 · Cya is a relatively small membrane protein (45 kDa for a monomer). The presence of an HD linking the TM domain with the catalytic domain of Cya makes this protein …

We have isolated cya-lac operon and protein fusions in Escherichia coli K-12, and we used these to study the regulation of cya, the structural gene for adenylate cyclase. Data obtained from …

2003年6月20日 · Catalyzes the formation of the second messenger cAMP from ATP. Its transcript is probably degraded by endoribonuclease LS (rnlA), decreasing cAMP levels and the …

The results indicate that cAMP-CRP is a negative regulator of cya expression at the level of transcription. We constructed cya-lac protein and operon fusion plasmids in vitro. The effect of …

It is shown that cya and crp mutations completely release the influence of the regulatory gene cytR on the activity of uridine phosphorylase (udp gene) and thymidine phosphorylase (tpp …

Cloning and analysis of the cya-5 gene reveal a long open reading frame capable of encoding a 1136 amino-acid protein. Sequence analysis suggests that the potential CYA-5 protein …

We have isolated cya-lac operon and protein fusions in Escherichia coli K-12, and we used these to study the regulation of cya, the structural gene for adenylate cyclase. Data obtained from …

The transcriptional regulation of cya by cAMP and its receptor protein (CRP) has been studied by S1 nuclease and RNA dot blot assays. The crp- Escherichia coli cells were shown to produce …