DBCO group can exclusively react with azide-tagged molecules or biomolecules to form a stable triazole. The click chemistry is also known as strain promoted alkyne-azide cycloaddition (SPAAC), DBCO reagent has become widely used in …

2023年2月23日 · DBCO (二苯并环辛炔, Dibenzocyclooctyne) 试剂是用于应变促进的炔叠氮化物环加成（SPAAC）的最具反应性的环炔之一，可实现无铜点击化学。 通常利用多肽中的氨基，巯基或羧基，把DBCO连接到多肽上， DBCO用于无需铜离子催化的叠氮-炔环加成 (SPAAC) 反应，该反应的二级反应动力学常数可达到 1。 DBCO 系列产品包括可用于进一步连接的 DBCO-acid、DBCO-amine、DBCO-PEG-COOH、DBCO-PEG-amine，以及 DBCO 标记的 Biotin、荧光染 …

The novel Copper-free Click Chemistry is based on the reaction of a diarylcyclooctyne moiety (DBCO, or ADIBO) with an azide-labeled reaction partner, known as strain-promoted alkyne azide cycloaddition (SPAAC). This new

2015年12月21日 · We demonstrate that site-specific incorporation of pAMF facilitates near complete conjugation of a DBCO-PEG-monomethyl auristatin (DBCO-PEG-MMAF) drug to the tumor-specific, Her2-binding IgG Trastuzumab using strain-promoted azide-alkyne cycloaddn.

When working with oYo-Link DBCO (Catalog #: AT3003), you must perform the click chemistry coupling reactions with the Azide-labeled molecule of interest first, prior to photo-crosslinking to an antibody. UV illumination will damage the DBCO activity and thus, antibody conjugation should only be performed after the click-reaction.

2020年11月2日 · Strain-promoted azide-alkyne cycloaddition using dibenzoazacyclooctyne (DBCO) is widely applied in copper-free bioorthogonal reactions. Reported here is the efficient acid-promoted rearrangement and silver-catalyzed amidation of DBCO, which alters its click reactivity robustly.

In order to conjugate antibodies, proteins and peptides containing dibenzocyclooctyne (DBCO) to surface reactive liposomes containing azide using fast, efficient and high yield click chemistry the molecule has to be modified. DBCO labeling can be done via C-terminus, N-terminus or through sulfhydryl group (e.g.

Strain-Promoted Azide-Alkyne Cycloaddition (SPAAC), also known as the copper-free click reaction, is a bioorthogonal method that allows for the efficient and exclusive reaction between cyclooctynes like dibenzocyclooctynes (DBCO) and azides.

DBCO (二苯并环辛炔, Dibenzocyclooctyne) 用于无需铜离子催化的叠氮-炔环加成 (SPAAC) 反应，该反应的二级反应动力学常数可达到 1。 DBCO 系列产品包括可用于进一步连接的 DBCO-acid、DBCO-amine、DBCO-PEG-COOH、DBCO-PEG-amine，以及 DBCO 标记的 Biotin、荧光染料和生化小分子等。

TAMRA azide, 5-isomer 的叠氮基团使其能够与炔烃、DBCO 和 BCN 进行铜催化的点击化学反应。 它含有 Azide 基团，可以和含有 Alkyne 基团的分子发生铜催化的叠氮-炔环加成反应（CuAAc)。