DBCO-PEG4-Biotin is a versatile biotinylation reagent used for the introduction of a biotin moiety to azide-labeled biomolecules via copper-free strain-promoted alkyne-azide click chemistry (SPAAC) reaction [1].

DBCO-PEG4-Biotin 是一种用途广泛的生物素酰化试剂，能通过无铜催化烷基叠氮化反应 (SPAAC) 将生物素的一部分引入叠氮标记的生物分子中。 DBCO-PEG4-Biotin 是一种点击化学试剂。

Typical reaction times are up to 4 hours, but longer incubation periods should be used if concentration is low or if low labeling is observed. Product is most efficient when first dissolved in a water miscible organic solvent and then diluted in aqueous reaction buffer when needed. DBCO-PEG4-Biotin is soluble in aqueous buffers up to 0.35 mM.

DBCO-Biotin is an alkyl chain-based PROTAC linker that can be used in the synthesis of PROTACs. DBCO-Biotin is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.

DBCO-Biotin | C28H30N4O3S | CID 121235600 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.

EZ-Link Biotin-PEG12-DBCO is a biotinylation reagent that contains a dibenzylcyclooctyne (DBCO) group for copper-free click chemistry attachment to an azide-labeled molecule, enabling the attachment of biotin using a bioorthogonal approach.

A biotin probe for copper-free click chemistry reaction, Biotin-PEG4-DBCO reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures. Often a reagent of choice in live cell surface glycans and lipids studies where the presence of cytotoxic copper catalyst is not acceptable.

2025年1月17日 · 生物分子标记 ：DBCO-Biotin可用于将生物素标记到蛋白质、核酸和脂质等生物分子上，实现亲和纯化、荧光成像和药物输送等多种应用。 生物医学研究 ：在生物医学研究中，该化合物可用于追踪和检测生物分子在体内的分布和动态变化，为疾病诊断和治疗提供 ...

2024年11月1日 · DBCO-Biotin结合了DBCO的反应活性和生物素的亲和性，可用于生物分子的纯化、检测和成像。 功能与应用： 该化合物可用于亲和层析、流式细胞术、免疫印迹等实验中，用于分离、检测和纯化含有亲和标签（如链霉亲和素）的生物分子。

2024年4月18日 · 在细胞生物学领域，DBCO-Biotin可用于标记细胞膜上的受体或细胞内的分子，揭示细胞内部信号传导的复杂网络。同时，Biotin作为一种强亲和力的配体，能够与多种生物分子如蛋白质、核酸等结合，进一步扩大了DBCO-Biotin的应用范围。_dbco-biotin蛋白标记后如何检测