The extracellular acidification rates (ECARs), which are linked to the glycolytic flux, did not result in significant changes following the DCA treatment of PANC-1, while inhibition was observed for the basal ECAR in BXPC-3 at the higher concentration of DCA and dose dependently for the glycolytic capacity (Figure 3E,F).

We found that DCA promoted OCR (OXPHOS) and partially inhibited ECAR (aerobic glycolysis) in platelets in response to suboptimal doses of convulxin. On the other hand, DCA promoted OCR in platelets in response to suboptimal doses of thrombin or ADP but surprisingly did not have any effect on ECAR.

2014年3月11日 · As measures of glycolytic parameters, the changes in the extracellular acidification rate (ECAR) were monitored in response to the sequential administration of d-glucose, oligomycin, and 2-deoxy-d-glucose (2-DG) to assess glycolysis, maximal glycolytic capacity, and glycolytic reserve capacity, respectively (Figure 5A). Immediately after ...

2021年4月30日 · Recently, we found evidence for synergism between dichloroacetate (DCA), a pyruvate dehydrogenase kinase inhibitor, and the HIF-1α inhibitor PX-478. In this study, we aimed to analyse this synergism in cell lines of different cancer types and to identify the underlying biochemical mechanisms.

为了检查 pdhk 抑制对巨噬细胞 nlrp3 炎性体激活的影响，作者使用了两种 pdhk 药理学抑制剂：dca 和 jx06。dca 通过与丙酮酸激酶口袋结合来阻断所有四种 pdhk 亚型。相比之下，jx06 通过与 atp 袋内的 cys240 形成二硫键优先阻断 pdhk 同种型。

用海马代谢生物分析仪测定细胞外酸化率（ecar），结果显示只有wt hk2细胞的糖酵解活性显著增加。 WT HK2细胞的糖酵解活性也能显著诱导乳酸生成和细胞乳酸水平 。

2014年5月1日 · When oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were measured by XF24 analyzer, ratio of OCR/ECAR was significantly increased by DCA treatment (p = 0.000), representing increased mitochondrial respiration, but decreased by metformin (p = 0.009) than the control.

2018年6月30日 · The oxygen consumption rate (OCR) and extra-cellular acidification rate (ECAR) were measured by Seahorse Technology. Ultra-low attachment plates were used to form spheroids. In vivo, DCA was administered to BXPC3-luc tumor-bearing nude mice.

2023年9月1日 · Our findings suggest that DCA effectively decreases the ECAR and increases the OCR in M. massiliense-infected macrophages. M. massiliense infection markedly increases the AMP/ATP ratio, activates AMPK phosphorylation, and induces autophagy. AMPK acts as a metabolic checkpoint that inhibits cell growth under nutrient-deprived conditions.

2022年3月29日 · Melanoma is characterized by high glucose uptake, partially mediated through elevated pyruvate dehydrogenase kinase (PDK), making PDK a potential treatment target in melanoma. We aimed to reduce glucose uptake in melanoma cell lines through PDK inhibitors dichloroacetate (DCA) and AZD7545 and throug …