Aim: To investigate the effects of metformin, dichloroacetate (DCA), and memantine on T98G and U87-MG human glioblastoma (GBM) cells to target tumor cell metabolism in a multi-directional manner. Material and methods: IC50 levels for metformin, DCA, metformin+DCA and memantine were determined by MTT assay in T98G and U87-MG cells in vitro.

These data demonstrate that PP2A reactivation prevents several compensatory mitochondrial survival mechanisms. Importantly, DCA failed to reduce glycolysis in T98G cells. At the same time, DCA enhanced the phosphorylation of AKT in T98G cells (Fig. 5A,B), which could compensate for glycolysis inhibition.

In order to investigate if GH could assist inhibitors of OXPHOS to induce cell death, we treated T98G ( Figure 5 A), B16 (Supplementary Figure S2A), and MDA-MB-231 (Supplementary Figure S2B) for...

Transfection of miR-548c-3p inhibited cell proliferation by inducing G 1 cell cycle arrest and also inhibited the migration of the T98G cells in vitro. Furthermore, a bioinformatic algorithm and a luciferase reporter assay identified proto-oncogene c-Myb (c …

To verify whether the synergistic effect between compound 3b (IC50, 2 µM) and R8-PNA-a221 can also occur in TMZ-resistant T98G cells, the protocol employed on U251 cells and presented in Figs. 6-8 was used. The results shown in Figs. 9 and 10 demonstrated that a synergistic effect between compound 3b and R8-PNA-a221 was also observed on T98G ...

T98G cells constitute a human glioblastoma cell line generally used in biomedical research. Particularly, these cells are utilized in brain cancer studies to investigate crucial cells and molecular factors contributing to it. Moreover, they help researchers screen and test new anti-cancer drugs that may aid in drug development.

Metformin and metformin-DCA combination decreased Casp-3 protein expression in T98G cells ( Figure 2 ), whereas Casp-3 protein expression was increased in all treatments (metformin, DCA,...

t98g细胞是一种合适的转染宿主，可用于神经科学和细胞周期研究。 物种：人，白人；男性，61岁 组织：脑；疾病：多形性胶质母细胞瘤 细胞来源：新引进 完全培养液配方：见下方备注。 批次/冻存日期：详见冻存管/培养瓶标识 参考传代比例：1:3-1:4

t98g人脑胶质瘤细胞是一种成纤维样细胞，从61岁的白人多形性胶质母细胞瘤男性患者的大脑中分离出来。 该细胞系由GH Stein保藏，可用于神经科学研究。

2019年8月1日 · We assessed the phenotypic characterization of the T98 G cells using spheroids. The invasion of the T98 G spheroids is collective with sporadic proliferation. The T98 G cells can be used as a control Glioblastoma cell line. Major Glioblastoma’s hallmarks include proliferation, invasion and heterogeneity.