Section of cortex of human kidney. The distal convoluted tubule (DCT) is a portion of kidney nephron between the loop of Henle and the collecting tubule. It is partly responsible for the regulation of potassium, sodium, calcium, and pH.

The distal convoluted tubule (DCT) is a short nephron segment, interposed between the macula densa and collecting duct. Even though it is short, it plays a key role in regulating extracellular fluid volume and electrolyte homeostasis. DCT cells are rich in mitochondria, and possess the highest densi …

2023年8月22日 · Late DCT and CD. There are two main cell types in this region: principal cells and intercalated cells. Principal Cells. Principal cells make up the majority of the tubular cells. They are mainly involved in the uptake of sodium ions and the extrusion of potassium ions.

2024年2月29日 · The distal convoluted tubule (DCT) is the tortuous, final segment of the renal tubule of the nephron, situated between the nephron loop (of Henle) and the connecting tubules. It is located within the cortex of the kidney, and is a direct continuation of the distal straight tubule (a.k.a. thick ascending limb of nephron loop).

The cells of the DCT contain an extensive basolateral membrane system and are mitochondria-rich, indicating high transport activity of the Na +-K +-ATPase. CNT cells, in contrast, contain fewer mitochondria and basolateral membrane invaginations, suggesting that they are less metabolically active.

2024年3月4日 · Notably, the review identified two studies that introduced novel immortalised human DCT cell lines developed from primary DCT cells. This paper provides a detailed account of the lineage of each cell model, their prevalent culture conditions, and the frequency and nature of gene transfections—both wild type and mutant—conducted in these models.

209/MDCT is a continuous line of distal convoluted tubule (DCT) cells expressing a phenotype including NaCl cotransporter (Slc12a3) and PTH receptors. The DCT is a primary site for regulation of blood pressure by controlling NaCl absorption and for calcium homeostasis by parathyroid hormone.

本综述探讨了人类细胞模型在肾脏生理学研究中的关键作用，特别关注远曲小管 (dct)。 它旨在提供与常用 DCT 细胞模型相关的起源、培养实践和遗传研究的全面总结。

This review presents an overview of cell models used to study the DCT and provides an outlook on kidney organoids and tubuloids as model for DCT (patho)physiology.

2011年7月1日 · The DCT is comprised almost entirely of a unique cell type, the DCT cell (Figure 16), although occasionally intercalated cells (ICs) are present. DCT cells have deep tight junctions, basolateral invaginations, many long mitochondria extending apically from the basolateral cell membrane, and short blunt apical microvilli ( Crayen and Thoenes 1975 ).