Impact of novel detergents on membrane protein studies
2022年4月14日 · Here, we provide the details of 23 different classes of novel detergents with particular focus on OGNG, LMNG, and GDN, which have been widely applied to the …
Lauryl maltose neopentyl glycol (Synonyms: 月桂基麦芽糖新戊二醇; LMNG)
Lauryl maltose neopentyl glycol (LMNG) is a detergent that can solubilize and stabilize membrane proteins. Lauryl maltose neopentyl glycol extracts integral membrane proteins from …
Detergent的性质及其在膜蛋白中的应用 - 知乎 - 知乎专栏
低cmc去垢剂一般通过疏水珠吸附除去，例如ddm。 还可以通过镍柱纯化，当膜蛋白结合到柱材料后，换含有另一种去垢剂溶液的缓冲即可。 4.2膜蛋白的鉴定
Quantification of Detergents Complexed with Membrane Proteins
2017年2月8日 · We tested the method with various detergents used for structural and functional biology, including (Fig. 1b) sugar derivatives such as n-dodecyl-β-D-maltoside (DDM), n-octyl …
Assemblies of lauryl maltose neopentyl glycol (LMNG) and LMNG ...
2019年5月1日 · Both SpNOX and BmrA display an increased specific activity in LMNG compared to DDM. The four proteins form LMNG complexes with their usual quaternary structure and …
An Overview of the Top Ten Detergents Used for Membrane …
2017年7月1日 · The most commonly used detergent in both categories (purification and crystallization) is n-Dodecyl-β-D-Maltopyranoside (DDM) (40.6% and 36.3% respectively), …
Universal Pretreatment Development for Low-input Proteomics …
2024年4月1日 · In this study, we have developed a lauryl maltose neopentyl glycol (LMNG)-assisted sample preparation (LASP) method. This method effectively reduces protein and …
十二烷基麦芽糖新戊二醇（LMNG）和LMNG溶解膜蛋白的集合体 …
与DDM相比，Apo和钒酸盐抑制形式的BmrA在LMNG中的有限蛋白水解动力学降低。 与DDM相比，SpNOX和BmrA在LMNG中均显示出增加的比活。 这四种蛋白质以其通常的四级结构和通常 …
High-Purity Maltoside Detergents - 赛默飞世尔科技公司
Lauryl Maltose Neopentyl Glycol (LMNG) is an amphiphilic detergent ideally suited to improve solubilization of integral membrane proteins while maintaining structural integrity and activity. …
Detergents and alternatives in cryo-EM studies of membrane …
Detergents with lower CMC are desirable in cryo-EM studies, such as GDN and LMNG, for which can minimize the detergent concentration while keep the protein soluble. Here I consider the …