A simple, sensitive and quick HPLC method was developed for the determination of ketoprofen in cell culture media (EMEM, DMEM, RPMI). Separation was performed using a gradient on the C18 column with a mobile phase of acetonitrile and miliQ water acidified by 0.1 % (v/v) formic acid.

A new pre-column derivative high-performance liquid chromatography (HPLC) method for determination of d-glucose with 3-O-methyl-d-glucose (3-OMG) as the internal standard was developed and validated in order to study the gluconeogenesis in HepG2 cells. Samples were derivatized with 1-phenyl-3-methy- …

2020年4月8日 · A stability-indicating RP-HPLC assay method for the determination of NAC and Di-NAC in the cell culture media has been developed. The proposed method showed good linearity for NAC (R = 1.00) and Di-NAC (R = 1.00), accuracy, precision, specificity and system suitability results within the acceptance criteria.

Pre-column fluorescent derivatization has been used for the fast quantification of amino acids using high-performance liquid chromatography (HPLC) systems. However, it generally requires an offline in-vial derivatization process with multiple ...

High-performance liquid chromatography (HPLC) has been used previously to quantify NO (15) due to its high sensitivity (16) and was identified as a potentially more useful...

2023年11月13日 · A simple, sensitive and quick HPLC method was developed for the determination of ketoprofen in cell culture media (EMEM, DMEM, RPMI). Separation was performed using a gradient on the C18 column...

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.

dmem/ham’s f-12就是一种无血清培养基，它适合哺乳动物细胞生长，包括mdck、胶质细胞、成纤维细胞、人内皮细胞和大鼠成纤维细胞。dmem提供高浓度的氨基酸、葡萄糖和维生素，ham’s f12提供锌、腐胺、次黄嘌呤和胸苷。可添加fbs和hepes等缓冲液补偿损失的缓冲能力。

DMEM is a modification of Basal Medium Eagle (BME) that contains a four-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. The original DMEM formula composition contains 1000 mg/L of glucose and was first reported for culturing embryonic mouse cells.

2023年12月27日 · DMEM（Dulbecco's Modified Eagle Medium）培养基作为细胞培养中的关键组成部分，在科学研究中发挥着不可替代的作用。 然而，在使用 DMEM培养基的过程中，研究人员常常会面临一系列问题，这些问题可能对实验的…