A simple, sensitive and quick HPLC method was developed for the determination of ketoprofen in cell culture media (EMEM, DMEM, RPMI). Separation was performed using a gradient on the C18 column with a mobile phase of acetonitrile and miliQ water acidified by 0.1 % (v/v) formic acid.

A new pre-column derivative high-performance liquid chromatography (HPLC) method for determination of d-glucose with 3-O-methyl-d-glucose (3-OMG) as the internal standard was developed and validated in order to study the gluconeogenesis in HepG2 cells. Samples were derivatized with 1-phenyl-3-methy- …

Apr 8, 2020 · A stability-indicating RP-HPLC assay method for the determination of NAC and Di-NAC in the cell culture media has been developed. The proposed method showed good linearity for NAC (R = 1.00) and Di-NAC (R = 1.00), accuracy, precision, specificity and system suitability results within the acceptance criteria.

Nov 13, 2023 · A simple, sensitive and quick HPLC method was developed for the determination of ketoprofen in cell culture media (EMEM, DMEM, RPMI). Separation was performed using a gradient on the C18 column...

High-performance liquid chromatography (HPLC) has been used previously to quantify NO (15) due to its high sensitivity (16) and was identified as a potentially more useful...

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.

DMEM是一种含各种氨基酸和葡萄糖的培养基，是在MEM培养基的基础上研制的。 与MEM比较增加了各种成分用量，同时又分为高糖型（高于4500mg/L）和低糖型（低于1000mg/L）。

Dec 27, 2023 · DMEM（Dulbecco's Modified Eagle Medium）培养基作为细胞培养中的关键组成部分，在科学研究中发挥着不可替代的作用。 然而，在使用 DMEM培养基的过程中，研究人员常常会面临一系列问题，这些问题可能对实验的…

Sep 21, 2023 · DMEM (Dulbecco 's Modified Eagle's Medium)是一种最广泛应用的细胞培养基之一，它是由 Eagle 于1959年开发出来的，后由 Dulbecco 进行了改良。 DMEM主要适用于肝脏、肾脏、肺、心脏等多种类型的哺乳动物细胞系的培养。 DMEM中含有较高浓度的葡萄糖（4500mg/L）、氨基酸、维生素和微量元素等营养物质。 此外，DMEM还添加了丙酮酸和乳酸等缓冲剂，能够保持细胞在较宽的pH范围内正常生长。 RPMI-1640 (Roswell Park Memorial …

alphaMEM和DMEM之间的主要区别在于，alphaMEM是一种含有较少葡萄糖、氨基酸和维生素的哺乳动物细胞培养基，而DMEM是一种含有较多葡萄糖、氨基酸和维生素的哺乳动物细胞培养基。