We previously demonstrated that DMF is capable of raising GSH levels even when glutathione synthesis is inhibited, suggesting enhanced GSH recycling. Here, we found that DMF indeed induces glutathione reductase (GSR), a homodimeric flavoprotein that catalyzes GSSG reduction to GSH by using NADPH as a reducing cofactor.

Key results: DMF but not MMF induced cell necroptosis, as demonstrated by the pharmacological tool necrostatin-1, transmission electron microscopy, LDH and HMGB1 release in CT26 cells. The DMF-induced decrease in cellular GSH levels as well as cell viability and increase in reactive oxygen species (ROS) were inhibited by co-treatment with GSH ...

Dimethyl fumarate (DMF) 是一种具有口服活性且可透过血脑屏障的 Nrf2 激活剂，可诱导抗氧化剂基因表达上调。 Dimethyl fumarate 通过 GSH 耗竭/ ROS 升高/MAPKs 激活途径诱导结肠癌细胞坏死，并诱导细胞自噬 ( autophagy )。

2024年11月22日 · Our findings indicate that DMF increases levels of glutathione (GSH), glutamate cysteine ligase modifier subunit (GCLM), and nuclear Nrf2 in SH-SY5Y cells. Importantly, DMF pretreatment significantly reduced the accumulation of …

通过与GSH和N-乙酰半胱氨酸（NAC）共同处理CT26细胞，可以抑制DMF诱导的细胞GSH水平降低以及细胞活力和活性氧（ROS）升高。 DMF激活CT26细胞中的JNK，p38和ERK MAPK，JNK，p38和ERK抑制剂部分逆转了DMF诱导的细胞活力下降。 GSH或NAC处理抑制了CT26细胞中DMF诱导的JNK，p38和ERK活化。 DMF而不是MMF会增加SGC-7901，HCT116，HT29和CT26癌细胞中的自噬反应，但自噬抑制作用并不能阻止DMF诱导的 …

2024年3月18日 · Dimethyl fumarate (DMF) is an anti-inflammatory and immunoregulatory drug used to treat patients with multiple sclerosis and psoriasis in which it blocks aberrant NF-κB pathways and...

2015年1月23日 · Here, we show that the accumulation of fumarate caused by the inactivation of FH leads to oxidative stress that is mediated by the formation of succinicGSH, a covalent adduct between fumarate and...

2015年5月8日 · We found that DMF but not MMF induced necroptosis in colon cancer cells by depleting cellular GSH and subsequently activating MAPKs. We propose that DMF has the potential to be an anticancer drug provided an appropriate drug delivery system can …

2021年9月9日 · In this study, we demonstrate a robust antilymphoma effect of DMF on both major DLBCL subtypes. DMF inhibited NF-κB and JAK/STAT3 survival signaling in ABC DLBCL, and it efficiently induced ferroptotic cell death in GCB DLBCL cells.

2024年11月22日 · Our findings indicate that DMF increases levels of glutathione (GSH), glutamate cysteine ligase modifier subunit (GCLM), and nuclear Nrf2 in SH-SY5Y cells. Importantly, DMF pretreatment significantly reduced the accumulation of …