2014年10月27日 · A single-nucleotide polymorphism (SNP) detection method was developed by combining single-base primer extension and salt-induced aggregation of gold nanoparticles densely functionalized with double-stranded DNA (dsDNA-AuNP).

Single unpaired nucleotides at the end of double-stranded nucleic acids, termed dangling ends, can contribute to duplex stability. Umbrella sampling free energy simulations of dangling cytosine and guanine nucleotides at the end of duplex and single stranded RNA and DNA molecules have been used to i …

2000年5月1日 · In this work, we report the thermodynamic contribution to duplex formation of the 32 DNA dangling ends with a closing Watson–Crick base pair. The available literature data indicate that dangling ends can significantly stabilize duplexes (8 – 11).

2014年12月22日 · A single-nucleotide polymorphism (SNP) detection method was developed by combining single-base primer extension and salt-induced aggregation of gold nanoparticles densely functionalized with double-stranded DNA (dsDNA-AuNP). The dsDNA-AuNPs undergo rapid aggregation in a medium of high ionic strengt …

2005年2月11日 · Single and dual-labeled fluorescent oligodeoxynucleotides are used in many molecular biology applications. We investigated the effects of commonly used fluorescent dyes and quenchers on the thermodynamic stability of a model probe–target DNA duplex. We demonstrate that those effects can be significant.

In this paper, we report the thermodynamic analysis of 3′T and 3′TT DNA dangling ends on model siRNA duplexes and their influence on siRNA stability depending on type and orientation of the closing Watson-Crick RNA base pair, i.e. A-U, U-A, G-C, or C-G. This data can now be used to improve design of siRNA for therapeutic uses. 2.

2005年3月17日 · By acting as a hydrophobic cap on the terminal of the DNA or RNA duplex, the dangling-end residue restricts the bulk water access to the terminal basepair, thereby providing it with a microenvironment devoid of water, which consequently enhances its thermodynamic stability, making it energetically comparable to the corresponding internal basepair.

2004年2月2日 · Remarkable stabilization of DNA triplex formation by the dangling residues was accomplished by employing novel nucleoside–aromatic ring conjugates as the dangling residues.

2024年6月21日 · Within a DNA strand, this fluorophore demonstrates characteristic decreases in quantum yield and emission decay lifetime that vary sensitively with base sequence, temp., and helix conformation but that are accompanied by only small changes in emission wavelength.

2000年5月1日 · The thermodynamic contributions to duplex formation of all 32 possible single-nucleotide dangling ends on a Watson-Crick pair are reported. In most instances, dangling ends are stabilizing with free energy contributions ranging from +0.48 (GT(A)) to-0.96 kcal/mol ().