Photolyases bind complementary DNA strands and break certain types of pyrimidine dimers that arise when a pair of thymine or cytosine bases on the same strand of DNA become covalently linked.

The photolyase enzyme reactivates DNA by direct repair of T>T lesions. It binds to the damaged DNA site, absorbs a (near UV-visible) photon, restores the pyrimidines to their monomeric forms, and dissociates from the substrate.

We then systematically summarize the detailed repair dynamics of damaged DNA by photolyases and a biomimetic system through resolving all elementary steps on ultrafast timescales, including multiple intermolecular electron- and proton-transfer reactions and bond-breaking and …

2023年11月30日 · Two teams of researchers have uncovered microscopic details of how a protein called photolyase uses light to repair DNA. The discovery could help develop sustainable technologies for chemical...

By capturing the excised oligomers, we have generated an excision repair map of the whole human genome. Circadian Clock is an innate timekeeping molecular mechanism that maintains daily rhythmicity in biochemical, physiological and behavioral functions independent of …

The enzyme first binds (in the dark) to UV-damaged DNA to form an enzyme-substrate complex. Then, in a light-dependent step, the dimer is split and photolyase dissociates from the repaired DNA. In contrast to other repair pathways like nucleotide excision repair (NER), which are

Photolyase, a photomachine discovered half a century ago for repair of sun-induced DNA damage of cyclobutane pyrim-idine dimers (CPDs) and pyrimidine (6-4) pyrimidone photo-products (6-4PPs), has been characterized extensively in biochemistry …

2007年5月22日 · Photolyase uses light energy to split UV-induced cyclobutane pyrimidine dimers in damaged DNA. This photoenzyme encompasses a series of elementary dynamical processes during repair function from early photoinitiation by a photoantenna molecule to enhance repair efficiency, to in vitro photoreduction through aromatic residues to reconvert the ...

Photolyases, a ubiquitous class of flavoproteins, use blue light to repair DNA photolesions. In this work, we determined the structural mechanism of the photolyase-catalyzed repair of a cyclobutane pyrimidine dimer (CPD) lesion using time-resolved serial femtosecond crystallography (TR-SFX).

Photolyase targets UV-induced DNA damage by recognizing pyrimidine dimers formed from adjacent thymine or cytosine bases. Once the enzyme binds to the damaged site, it absorbs light energy, which enables it to cleave the bonds between the dimerized bases.