DNase I, (RNase-free) is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5´-phosphorylated and 3´-hydroxylated ends. DNase I acts on single- and double-stranded DNA, chromatin and RNA:DNA hybrids.

Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups. The enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ ions.

DNase I is suitable for removing DNA from protein preparations, nick translating DNA, and generating random fragments for dideoxy sequencing. NOTE: for removing DNA from RNA preparations, use Amplification Grade DNase I (Cat. No. 18068-015).

A powerful research tool for DNA manipulations, DNase I is used in a range of molecular biology applications. Some of its uses include: 1. Degradation of contaminating DNA after RNA isolation, 2. "Clean-up" of RNA prior to RT-PCR and after in vitro transcription, 3. Identification of protein binding sequences on DNA (DNase I footprinting), 4.

Jun 1, 2001 · In this review, available structural data of deoxyribonucleases I (DNases I) from several mammalian species, hen, snake and frog are summarized. Comparative studies on enzymatic and immunological properties and glycosylation are discussed, and several evolutionary conclusions are presented.

DNase I，即Deoxyribonuclease I，中文名称为脱氧核糖核酸酶I，是一种可以消化单链或双链DNA产生单脱氧核苷酸或单链或双链的寡脱氧核苷酸的核酸内切酶。 DNase I水解单链或双链DNA后的产物，5'端为磷酸基团，3'端为羟基。 DNase I活性依赖于钙离子，并能被镁离子或二价锰离子激活。 镁离子存在条件下，DNase I可随机剪切双链DNA的任意位点；二价锰离子存在 …

In this review, available structural data of deoxyribonucleases I (DNases I) from several mammalian species, hen, snake and frog are summarized. Comparative studies on enzymatic and immunological properties and glycosylation are discussed, and several evolutionary conclusions are presented.

Recombinant DNase I (rDNase I) is a recombinant form of bovine DNase I purified from an overexpressing host. rDNase I is bovine-free. In addition, the host expressing rDNase I contains 107-fold less endogenous RNase activity than pancreatic tissues, the source for bovine DNase I.

Because DNase I poorly cleaves intact chromatin and G-actin (an abundant cellular protein) inhibits DNase I activity, efficient chromatin cleavage by DNase I requires at least exposure of DNA by degrading or replacing DNA-binding proteins, such as core histones or linker histone H1.

DNase I（Deoxyribonuclease I）中文名称为 脱氧核糖核酸酶 I，是一种可以消化单链或 双链 DNA产生单 脱氧核苷酸 或单链或双链的 寡脱氧核苷酸 的 核酸内切酶。 DNase I水解单链或双链 DNA 后的产物，5'端为 磷酸 基团，3'端为羟基。 DNase I活性依赖于 钙离子，并能被 镁离子 或二价锰离子激活。 镁离子存在条件下，DNase I可随机剪切双链DNA的任意位点；二价锰离子存 …