2018年8月18日 · We established a Source Animal (barrier) Facility (SAF) for generating designated pathogen-free (DPF) pigs to serve as donors of viable organs, tissues, or cells for xenotransplantation into clinical patients.

2023年4月3日 · The DPF standards for pig husbandry can help eliminate specific pathogens in donor pigs. Porcine endogenous retroviruses (PERVs) are present in the genome of all pig strains. PERV are divided into three classes, PERV-A, PERV-B, and PERV-C.

Background: We established a Source Animal (barrier) Facility (SAF) for generating designated pathogen-free (DPF) pigs to serve as donors of viable organs, tissues, or cells for xenotransplantation into clinical patients. This facility was populated with caesarian derived, colostrum deprived (CDCD) piglets, from sows of conventional-specific ...

We established a Source Animal (barrier) Facility (SAF) for generating designated pathogen‐free (DPF) pigs to serve as donors of viable organs, tissues, or cells for xenotransplantation into clinical patients.

2019年11月7日 · Source donor pigs fulfilling the Designated Pathogen-Free (DPF) status have been available from a closed colony by GMP(Good Manufacturing Practice) rigorous routines, operational SOPs and rigorous data retention. Above all are very important for the operation of GMP barrier facility for biosafety of DPF source pig.

Results: The DPF standards for pig husbandry eliminated specific pathogens in donor pigs. We have established a PERV‐C free, genetic information clean, DPF donor for xenotransplantation. A clinical trial of ten adult patients (9M:1F) with type 1 diabetes who received DPF porcine islet xeno-transplantation via the portal vein were performed.

2018年7月1日 · In order to generate designated pathogen-free (DPF) pigs to serve as donors for xenotransplantation into clinical patients, a new facility at the Spring Point Project, Minneapolis, Minnesota,...

Spring Point Project has selected a special strain of pigs with high islet cell yield for transplantation. Bacteria and viruses were removed from the pig herd, and the animals are housed in such a way so they remain free of such infectious agents – the designated pathogen-free (DPF) status.

2014年1月1日 · The effect of feeding pigs in a three-phase feeding (3PF) system or a daily-phase feeding (DPF) system on growth performance, body composition, and N and P excretions was studied on 8 pens of 10 pigs each.

We aimed to investigate whether the use of the newer bovine nervous tissue-free enzymes and a revised donor selection strategy could improve the islet graft function in the context of islet isolation with DPF pigs. Methods: Using 30 DPF pigs within a closed herd, we compared the islet yield of porcine islets isolated with Liberase PI (n = 11 ...