MMEJ作为替代末端连接的一种形式，只需要非常小的同源区域(5~25bp)进行修复，使得构建靶向载体更加容易。Takashi Yamamoto实验室利用MMEJ创建了CRISPR基因敲入的新方法，命名为PITCh (精确整合到目标染色体中)。

2019年4月4日 · NHEJ通路是大多数细胞中占主导地位的DSB修复通路。MMEJ通路通过末端切除来发现断裂两侧的微小同源序列，这些同源序列可用于模板化断裂端融合。PARP-1可通过MMEJ通路调控DSB修复。用PARP-1抑制剂rucaparib治疗细胞可降低DSB在MMEJ修复通路中的通量。

2017年1月13日 · MMEJ is a mutagenic DSB repair mechanism, which always associates with deletions flanking the break sites and contributes to chromosome translocations and rearrangements. Recent study indicated that MMEJ is used with appreciable frequency even when HR is available [13].

2022年12月19日 · The double-strand break (DSB) repair pathway called microhomology-mediated end-joining (MMEJ) is thought to be dependent on DNA polymerase theta (Polθ) and occur independently of nonhomologous...

Microhomology-mediated end joining (MMEJ) is a mutagenic double-strand break (DSB) repair mechanism that uses 1-16 nt of homology flanking the initiating DSB to align the ends for repair. MMEJ is associated with deletions and insertions that mark the original break site, as well as chromosome translocations.

This highly error-prone DSB repair pathway is termed microhomology-mediated end joining (MMEJ). Dissecting the mechanisms of MMEJ is of great interest because of its potential to destabilize the genome through gene deletions and chromosomal rearrangements in cells deficient in canonical repair pathways, including HR and C-NHEJ.

2023年7月20日 · We found that the combined inhibition of NHEJ by the K3753R mutation in DNA-PKcs and by Polθ V896* (stop codon introduction) in MMEJ results in DSB repair almost exclusively by HDR, while...

2025年2月1日 · This review discusses the different molecular players of HR, NHEJ, and MMEJ pathways that control the switch among the different DSB repair pathways. We also highlight the various functions of chromatin modifications in modulating repair response and how deregulated DNA damage repair response may promote oncogenic transformation.

dna双链断裂（dsb）是一种严重的dna损伤，如果不及时或不正确地修复，会导致基因组的不稳定性，进而引发癌症或其他疾病。 细胞拥有多种修复DSB的途径，其中最主要的两种是非同源末端连接修复（NHEJ）和同源重组修复（HR）。

This highly error-prone DSB repair pathway is termed microhomology-mediated end joining (MMEJ). Dissecting the mechanisms of MMEJ is of great interest because of its potential to destabilize the genome through gene deletions and chromosomal rearrangements in cells deficient in canonical repair pathways, including HR and C-NHEJ.