2019年10月1日 · DNA double strand breaks (DSBs) are the most toxic form of DNA damage. To prevent damage accumulating, mammalian cells have evolved a complex network, collectively referred to as the DNA damage response (DDR) to sense and repair DSBs (Box 1). Additionally, the DDR can also activate checkpoints that pause cell cycle progression to facilitate repair.

6 天之前 · In mammals, damaged rDNA is transcriptionally repressed by the kinase MST2 (STK3) and cohesin–human silencing hub (HUSH) complexes, thereby promoting DSB movement to nucleolar caps.

2022年1月4日 · Here, by analyzing the localization of DSB repair factors in the XY body and DSB foci, we demonstrate that pre-ribosomal RNA (pre-rRNA) mediates the recruitment of DSB repair factors around...

2025年2月20日 · 该研究基于体外表达和纯化的小鼠 SPO11-TOP6BL 复合体，首次在体外成功重构了 DNA 双链断裂 (Double-Strand Break, DSB) 的形成，为解析减数分裂同源重组机制建立了研究平台。

2020年9月9日 · Recent discoveries have significantly extended our understanding of how cells respond to DNA double-strand breaks (DSBs) in the nucleolus, and new kinases and multiple down-stream targets have been identified.

2011年8月7日 · DNA double strand breaks (DSBs) in repetitive sequences are a potent source of genomic instability, owing to the possibility of non-allelic homologous recombination (NAHR). Repetitive...

2015年10月13日 · Resolution of DNA double-strand breaks (DSBs) is essential for the suppression of genome instability. DSB repair in transcriptionally active genomic regions represents a unique challenge that is associated with ataxia telangiectasia mutated (ATM) kinase-mediated transcriptional silencing.

高拷贝核糖体 DNA (rDNA) 基因由 RNA 聚合酶 I (RNAAPI) 高度转录，本质上不稳定，经常会因转录复制冲突而发生 DNA 双链断裂 (DSB)。 当 DSB 发生时，RNAPI 被 ATM 信号传导沉默，rDNA DSB 易位到核仁外围，主要通过新形成的核仁帽中的同源重组进行修复。

2018年12月14日 · Our findings provide direct evidence for diRNA production at endogenous loci in mammalian cells and give insights into RNA processing at DSBs. 最近的研究表明，DNA双链断裂（DSBs）中会发生转录，DSB的转录本由Drosha和Dicer加工成损伤诱导的小RNA（diRNA），用于修复DNA。 但是，最近diRNA大多多数在报告构建体或重复序列当中被检测到，并且最近的报道针对它们在内源基因座的存在性也提出了质疑。 我们在遗传未受干扰的 …

2019年9月1日 · DNA double-strand breaks (DSBs) within rDNA induce both rDNA transcriptional repression and nucleolar segregation, but the link between the two events remains unclear. Here we found that DSBs induced on rDNA trigger transcriptional repression in a cohesin- and HUSH (human silencing hub) complex-dependent manner throughout the cell cycle.