rAAV2-retro通过逆行途径获得的转基因表达水平足以用于神经环路功能示踪，以及对神经元基因组的靶向操作。 因此，通过选择性监测和操作连接不同脑区的投射神经元，rAAV2-retro有望为神经环路如何促进大脑发挥功能提供深入见解，并可能为神经退行性疾病的 ...

• Retro fits to tiles where trim is added after tiling process is completed • Used where tile meets carpet, wooden floors etc. • Ideal for creating a stylish and professional finish • Can be used on floor and wall • Includes adhesive tape for easy application

Plasmid pAAV-mCherry-flex-dtA from Dr. Naoshige Uchida's lab contains the insert dtA and is published in Nature. 2014 May 15;509(7500):325-30. doi: 10.1038/nature13307. This plasmid is available through Addgene.

直到2016年，研究人员在AAV2的基础上，通过定向进化的方式改造出一种具有逆向示踪功能的AAV2突变体——AAV2-retro，该载体能够在小鼠大脑中进行有效的神经环路逆向标记，且其逆行示踪能力和效率与传统的逆行示踪剂相当。图4所示为rAAV2-retro的开发过程及应用 ...

These viral vector preparations were produced with the rAAV2-retro helper plasmid (Addgene #81070). This serotype permits robust retrograde access and transgene delivery to projection neurons. Specific classes of projection neurons can be targeted by using this serotype to deliver Cre-dependent transgenes in Cre transgenic lines.

• Retro fit trim can be added after tiling process is complete • Suitable for internal corners • Ideal for creating a stylish and professional finish • Includes adhesive tape for easy application

Retro fits to tiles where trim is to be added once the tiling process is completed. Designed to protect the edge of tiles; Ideal for creating a stylish and professional finish

为了排除抑制BLA输入mPFC产生镇痛作用涉及其他(平行)通路的可能性，通过双重注射AAV-mCherry- flex-dtA和Cav2-cre病毒选择性消除该通路中的投射。 这导致白喉毒素亚基 A (dtA)仅在连接两个注射的大脑区域的神经元中表达，因此被消融。

2022年7月27日 · Recently, engineering of the AAV2 capsid has generated an AAV variant, called rAAV2-retro, with exceptional retrograde functionality. This variant comprises a 10-mer peptide insertion at residue 587 and two point mutations (LADQDYTKTA + V708I + N382D).

2024年5月6日 · 直到 2016 年，研究人员在 AAV2 的基础上，通过定向进化的方式改造出一种具有逆向示踪功能的 AAV2 突变体——AAV2-retro，该载体能够在小鼠大脑中进行有效的神经环路逆向标记，且其逆行示踪能力和效率与传统的逆行示踪剂相当。