Dithiothreitol (DTT) is an organosulfur compound with the formula (CH(OH)CH 2 SH) 2. A colorless compound, it is classified as a dithiol and a diol. DTT is redox reagent also known as Cleland's reagent, after W. Wallace Cleland. [2] The reagent is commonly used in its racemic form. Its name derives from the four-carbon sugar, threose.

Thermo Scientific DTT (DL-Dithiothreitol; Clelands reagent) is used to stabilize enzymes and other proteins, which possess free sulfhydryl groups. It has been shown to restore activity lost by oxidation of these groups in vitro.

二硫苏糖醇（Dithiothreitol，简称为DTT）是一种小分子有机还原剂，化学式为C4H10O2S2。其还原状态下为线性分子，被氧化后变为包含二硫键的六元环状结构。二硫苏糖醇的名字衍生自苏糖（一种四碳单糖）。DTT的异构体为二硫赤糖醇（DTE），即DTT的C3-差向异构体。

A reagent commonly used in biochemical studies as a protective agent to prevent the oxidation of SH (thiol) groups and for reducing disulphides to dithiols. (2 R,3 R)-1,4-bis (sulfanyl)butane-2,3-diol. Computed by Lexichem TK 2.7.0 (PubChem release 2021.10.14) InChI=1S/C4H10O2S2/c5-3 (1-7)4 (6)2-8/h3-8H,1-2H2/t3-,4-/m0/s1.

Sep 11, 2024 · DTT (Dithiothreitol) is a “thiol reagent” that dissolves disulfide bonds between cysteine amino acids, potentially affecting both red cell antigens and antibodies. Other, less commonly used examples of thiol reagents are 2-mercaptoethanol (2-ME) and 2-aminoethylisothiouronium bromide (AET).

Dithiothreitol (DTT) is a disulfide (SH) reducing agent found in proteins and in the amino acid cysteine. It reduces a disulfide bond by forming a six-membrane ring and initiating a thiol-disulfide interchange. It is almost 7-fold stronger reducing agent than βME, hence efficient in …

DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel.

Thermo Scientific Pierce DTT (Dithiothreitol), No-Weigh format, is offered as 7.7 mg x 48-tube microplates for convenient preparation of 100 μL of 0.5 M single-use stocks for addition to protein samples for effective reduction of disulfide bonds.

pound 2,3-dihydroxy-1,4-dithiolbutane. DTT, also known as Cleland’s Reagent, is a cell-permeable, strong but mild reducing agent, with a low redox potential of -0.33 V. at pH 7 (optimal pH range 7.1 to 8.0). This allows it to reduce practically all …

Discrete trial training (DTT) is a technique used by practitioners of applied behavior analysis (ABA) that was developed by Ivar Lovaas at the University of California, Los Angeles (UCLA). DTT uses mass instruction and reinforcers that create clear contingencies to shape new skills. Often employed as an early intensive behavioral intervention (EIBI) for up to 25–40 hours per …