We discuss novel insight into the role and consequences of the phosphorylation of the translation initiation factor eIF2α in the context of stress responses and cell-cycle regulation. eIF2α is centrally located to regulate translation and its phosphorylation in response to different environmental challenges is one of the best characterized stres...

2023年8月21日 · Using a mammalian in vitro translation system, we found that increased levels of recombinant human eIF2A inhibit translation of multiple reporter mRNAs, including those that are translated by cognate and near-cognate start codons, and does so prior to start codon recognition. eIF2A also inhibited translation directed by all four types of cap-ind...

2020年10月7日 · We used a transgenic mouse harbouring both the non-phosphorylatable Ser51Ala mutant Eif2a gene (Eif2aA/A) and the wild-type Eif2a transgene driven by the CMV enhancer and chicken β-actin...

2017年3月9日 · Naïve cells or cells pretreated with 100 mM trehalose for 1 h were either treated with an ER stress inducer dithiothreitol (DTT, 1 mM) for 4 h or exposed to 43 °C heat shock (HS) for 1 h with ...

eIF2 起始复合体整合了各种各样应激相关的信号来调控全局和特异性 mRNA 翻译。 在适宜的条件下，eIF2 会结合 GTP 和 Met-tRNAi 形成三元复合体 (TC)，三元复合体随后结合 40S 核糖体亚基、eIF1、eIF1A、eIF5 和 eIF3，形成 43S 起始前复合体 (PIC)。 43S PIC 扫描 mRNA UTR 以寻找 AUG 起始密码子。 在识别 AUG 后，eIF2 在GTP酶活化蛋白 eIF5 的帮助下可将 GTP 水解为 GDP，并从 mRNA 上分解下来，以结合 60S 核糖体亚基并延长多肽链。 在 eIF5 作为 GDI 而 …

2020年6月8日 · Here we performed a systematic analysis of mammalian cells responding to a vast panel of autophagy modulators to determine their dependence on peIF2α and eIF2α kinases.

Treatment with 400 mM DTT, 200 mM Ars and 1 mM Tg has no effect on the total amount of eIF2a, while the phosphorylated form of this factor clearly increases in the presence of any of these...

2015年8月1日 · DTT-induced phosphorylation of eIF2α at Ser 51 was reduced in the hyper-O-GlcNAcylation condition (Fig. 2). Using 1DLC–MS/MS and mutagenesis analyses, we showed that eIF2α was directly O-GlcNAcylated at Ser 219, Thr 239, and Thr 241.

2017年1月1日 · Here, we report that eIF2A, an alternative tRNA i carrier, is responsible for the stress-resistant translation of c-Src mRNA. eIF2A facilitates tRNA i loading onto the 40S ribosomal subunit in a c-Src mRNA-dependent manner. And a direct interaction between eIF2A and a stem-loop structure (SL I) in the c-Src IRES is required for the c-Src IRES ...

reveal a noncanonical eIF2 signaling pathway that controls selective changes in axon translation and is required for neural wiring. Precise connectivity between neurons is needed to generate operative nervous systems.