2017年9月25日 · In my experience, DAPI is only semi-permeant to live cells. Some cell lines label well, others partially, others not at all. So I always recommend at least a light permeabilization to...

DAPI can be permeable to live cells. DAPI uptake by live cells can be prevented by optimizing concentration, adding it immediately prior to acquisition and controlling sample temperature, understanding that ideal conditions can vary across cell types.

DAPI is generally used to stain fixed cells since the dye is cell impermeant, although the stain will enter live cells when used at higher concentrations. For live-cell staining, Hoechst 33342 dye is a popular cell-permeant nuclear counterstain.

DAPI (pronounced 'DAPPY', /ˈdæpiː/), or 4′,6-diamidino-2-phenylindole, is a fluorescent stain that binds strongly to adenine – thymine -rich regions in DNA. It is used extensively in fluorescence microscopy.

DAPI is usually used to stain fixed/permeabilized cells whereas Hoechst stain can be used to stain live cells also. Both DAPI and Hoechst are cell permeable. The main difference is that the...

DAPI is another DNA dye frequently used for cell quantification. DAPI belongs to the indol substances (Figure 2a), and initially, it was synthesized by Dann et al. as a potentially active trypanocidal diamine [16,17].

Here, we tested different protocols for DAPI-PolyP detection in a range of samples with different levels of DAPI permeability, including subcellular fractions, free-living cells and cryosections of fixed tissues.

DAPI staining is particularly useful for identifying both live and dead cells, but it is often noted for its ability to stain dead cells more efficiently. This specificity arises from the permeability of the cell membrane .

2023年9月9日 · We have demonstrated that the addition of B 12 Br 12 2– to impermeable dicationic fluorescent DNA-staining dyes, such as DAPI, PI, and MG, which have been widely used for numerous years, imparts membrane permeability to introduce these dyes into living cells.

DAPI is a cell-permeable fluorescent compound that is able to stain the DNA of eukaryotic and prokaryotic cells by binding with high affinity to the minor groove of AT-rich DNA sequences.