2021年8月5日 · Murine fibroblast cell line L929 was used to study cell-substrate interplay on differentially crosslinked Gel hydrogels using MTT assay, Raman spectroscopic approach and Rhodamine-DAPI staining. The cells were maintained in DMEM supplemented with 10% (v/v) FBS and 1000 units/mL antibiotic (penicillin + streptomycin) at 5% CO2 and 37 °C in a ...

鬼笔环肽 （Phalloidin）是 细胞骨架 的染色神器，罗丹明标记的Phalloidin（TRITC- Phalloidin）可将细胞染成橙红色，DAPI与Phalloidin染色液是研究细胞形态变化时经常用到的两种 共染 的试剂，染色效果可见图1 [1]：A1-E1用TRITC-鬼笔环肽（橙红）染色的细胞骨架免疫荧光图；A2-E2用DAPI（蓝色）染色的细胞核免疫荧光图；A3-E3合并的L929细胞免疫荧光染色图。 图2 [2]是S100A16过表达或下调前后PDAC细胞形态的变化。 ※ 鬼笔环肽具有毒性，需小心操作（对 …

2021年8月5日 · In the present study, Raman spectroscopy assisted approach was used for biochemical evaluation of L929 fibroblast cells on diffrentially crosslinked Gel matrices. The physicomechanical properties of Gel-EDC and Gel-GTA were first evaluated to confirm successful fabrication and it was observed that all crosslinked samples showed excellent ...

L929 cell supernatant is commonly used to differentiate murine macrophages from bone marrow. The supernatant and its effect on macrophage phenotype was characterised by proteomics and the authors identified novel immunoregulatory proteins.

基于拉曼的方法通过识别关键的生物分子特征来帮助评估细胞-基质相互作用，回顾与裸对应物相比，l929 成纤维细胞在交联凝胶中表现出优异的生长和增殖动力学这一事实。

Actin filament staining of L929 cells with Alexafluor 488 phalloidin and 4′,6-diamidino-2-phenylindole (DAPI) for nucleus counterstaining. Scale bar 20 μm. Hydrophobic polymers, for their...

Dapi staining of l929 cells treated with different concentrations of Butterbur extract for 72 h. Bar is 50 µm. [...] In this study, we present initial data for the potential anti-cancer effects...

Ki-67-staining and DNA counterstain with DAPI were performed on murine fibroblasts with three different anti Ki-67 antibody clones and the respective secondary antibody for the SP-6 antibody ...

2021年8月5日 · Raman-based approach assisted in evaluation of cell-matrix interactions by identifying key biomolecular signatures retrospecting the fact that L929 fibroblast cells portrayed excellent growth and proliferation kinetics in crosslinked Gel as compared to its bare counterpart.

DAPI is a fluorescent stain that binds strongly to A-T-rich regions in DNA. DAPI and PI only inefficiently pass through an intact cell membrane and, therefore, preferentially stain dead cells.