聚酮合酶的催化机制及聚酮类化合物的组合生物合成 - 知乎
6-脱氧-红霉内酯B合成酶(6-deoxy-erythronolide B synthase,DEBS)是一种典型的mPKS(Fig.1),由DEBS1、DEBS2和DEBS3三条多肽构成,执行1个加载模块(loading didomain,LDD)和6个延伸 …
The DEBS paradigm for type I modular polyketide synthases and …
Nucleotide sequencing revealed that the PKS that produces the polyketide backbone of the antibiotic erythromycin, DEBS (for 6-deoxyerythronolide B synthase), contains a discrete …
Mapping the catalytic conformations of an assembly-line ... - Science
2021年11月4日 · Assembly-line polyketide synthases, such as the 6-deoxyerythronolide B synthase (DEBS), are large enzyme factories prized for their ability to produce specific and …
Programming of Erythromycin Biosynthesis by a Modular …
The biosynthetic intermediates never leave the polyketide synthase (PKS) but are passed along the DEBS assembly line from one acyl carrier protein (ACP) domain to the next. In fact, DEBS …
Enzymology of assembly line synthesis by modular polyketide
2023年3月13日 · The modular PKS DEBS from Saccharopolyspora erythraea, producing the erythromycin precursor 6-deoxyerythronolide, is composed of six modules that are distributed …
Combinatorial polyketide biosynthesis by de novo design and ...
2005年8月14日 · The paradigm PKS gene set for 6-deoxyerythronolide B synthase (DEBS; Fig. 1a), which builds 6-deoxyerythronolide B, the erythromycin aglycone, encodes six extender …
Chapter 6 The DEBS Paradigm for Type I Modular ... - ScienceDirect
2009年1月1日 · Nucleotide sequencing revealed that the PKS that produces the polyketide backbone of the antibiotic erythromycin, DEBS (for 6-deoxyerythronolide B synthase), contains …
中外学者Science最新发文：聚酮合酶（PKS）的结构新细节
学术论文解析了Saccharopolyspora erythraea生产红霉素装配线6-deoxyerythronolide B 合酶（DEBS）的冷冻电镜结构，揭示了其如何推动分子链的不断组装增长的理论支持。 自然界是 …
Advances in polyketide synthase structure and function
The structural analysis of many components of PKS, in particular for the modular type I 6-deoxyerythronilide B synthase (DEBS) involved in erythromycin biosynthesis, has provided …
Structure and Mechanism of Assembly Line Polyketide Synthases
Assembly line polyketide synthases (PKSs) are remarkable biosynthetic machines with considerable potential for structure-based engineering. Several types of protein-protein …