Decalcification describes the technique for removing minerals from bone or other calcified tissue so that good-quality paraffin sections can be prepared that will preserve all the essential microscopic elements. Decalcification is carried out after the specimen has been thoroughly fixed and prior to routine processing to paraffin.

2023年7月12日 · This document provides an overview of decalcification in histopathology. It discusses the need to decalcify bony tissue specimens to make them thin enough for microscopic examination. The key aspects of decalcification covered include the criteria for good decalcifying agents, factors that affect the process, common techniques and decalcifiers ...

How does the decalcification process work? Any tissue containing bone, teeth, or calcifications must be FULLY FIXED prior to decalcification. Decalcification must be performed such that the tissue can be sectioned in the gross room with a blade and/or in histology with a microtome.

After fixation tissues must be thoroughly washed and excess fixative should be removed before the specimen is subjected to decalcification. The tissue is cut into small pieces of 3 to 5 mm size. This helps in faster decalcification. The tissue is then suspended in …

2018年6月9日 · This chapter discusses the method of decalcification of bony and hard tissue for histopathology processing. Decalcification technique helps to remove calcium salt from the tissue without affecting the morphology of the tissue and staining.

2024年1月25日 · Specimens should be decalcified in hydrochloric acid/formic acid working solution 20 times their volume. Change to fresh solution each day until decalcification is complete. It may take 24 hours up to days or months depending on size of the specimens.

2022年4月2日 · DECALCIFICATION is the removal of calcium ions from a bone or calcified tissue through a histological process that makes them flexible and easier to cut it adjusts the hard substance of bones to the softness of paraffin embedding medium enables the histotechnologist to cut soft sections of the bone using the microtome, so that they can be ...

2011年9月26日 · In order to achieve optimal results when processing calcified tissues, it is important to determine the point at which decalcification is complete. While incomplete decalcification can lead to tissue distortions (and possibly a damaged microtome), over-decalcification causes problems with staining, in particular nuclear staining.

III. DECALCIFICATION Decalcification is the process of complete removal of calcium salt and mineral from the bone tissue following proper fixation (see Chapter 10). Without decalcification, sectioning is nearly impossible unless the specimen is embedded in a plastic medium and cut with a heavy-duty rotary microtome.

What is Decalcification? Decalcification describes the technique for removing calcium minerals from bone or other calcified tissue so paraffin sections can be sectioned. Decalcification is carried out between fixation and processing steps. Amount of unwanted calcium salts determines the decalcification method that must be used.