Decalcification describes the technique for removing minerals from bone or other calcified tissue so that good-quality paraffin sections can be prepared that will preserve all the essential microscopic elements. Decalcification is carried out after the specimen has been thoroughly fixed and prior to routine processing to paraffin.

How does the decalcification process work? Any tissue containing bone, teeth, or calcifications must be FULLY FIXED prior to decalcification. Decalcification must be performed such that the tissue can be sectioned in the gross room with a blade and/or in histology with a microtome.

Decalcification describes the technique for removing calcium minerals from bone or other calcified tissue so paraffin sections can be sectioned. Decalcification is carried out between fixation and processing steps. What is Decalcification?

2024年1月25日 · Specimens should be decalcified in hydrochloric acid/formic acid working solution 20 times their volume. Change to fresh solution each day until decalcification is complete. It may take 24 hours up to days or months depending on size of the specimens.

2024年1月25日 · EDTA is a chelating agent, and it can be made 10% solution with distilled water, pH 7.4. This is also the preferred solution for decalcifying bone material for transmission electron microscopy.

Decalcifying Solution-Lite is designed to be a universal effective decalcifying agent. It is intended for the decalcification of routine, immunohistochemical and bone marrow core specimens. Decalcifying Solution-Lite has been tailored to suit your specific lab routine.

This method is characterized by long decalcification time, less damage to bone tissue, better preservation of enzyme activity (alkaline phosphatase) and cell antigenicity. The slices can be used for histochemical and immunohistochemical analysis.

Acid Decalcification – This is the most commonly used method. Various acid solutions may be used alone or in combination with a neutralizer. The neutralizer helps in preventing the swelling of the cells. If tissue is left for long time in the solution, the tissue may be damaged. Yellow colour of nitric acid should be removed with urea.

Most of us already know that decalcification is the term used for removing mineral from bones or other calcified tissue, such as cartilage. This allows for proper press processing, and sectioning of specimens to be used for histological analysis.

A decalcifying solution formulated to decalcify bone and retain antigenicity for immunochemical studies. EDTA™ decalcifier is acid free to maintain nucleic acid integrity.