Decalcified sections are used for the examination of bone marrow and for the diagnosis of tumours, infections, or for other purposes. The specimens may be in the form of iliac crest trephines, or bone pieces removed at operation (such as femoral heads) or dissected from amputation specimens.

Bone decalcification is the softening of bones due to the removal of calcium ions, and can be performed as a histological technique to study bones and extract DNA. [1][2] This process also occurs naturally during bone development and growth, and when uninhibited, can cause diseases such as osteomalacia. [3]

The meaning of DECALCIFICATION is the removal or loss of calcium or calcium compounds (as from bones or soil).

Decalcification must be performed such that the tissue can be sectioned in the gross room with a blade and/or in histology with a microtome. Scant bony fragments (as in marrow margins) can be frozen fresh (without decal) as long as they are small and can be cut with the cryostat.

2015年8月24日 · It defines decalcification and outlines the structure of bone and teeth. Various decalcifying agents are described including strong acids, weak acids, and chelating agents. Factors that influence the rate of decalcification and techniques for determining when it is complete are also covered.

Most of us already know that decalcification is the term used for removing mineral from bones or other calcified tissue, such as cartilage. This allows for proper press processing, and sectioning of specimens to be used for histological analysis.

Decalcification describes the technique for removing calcium minerals from bone or other calcified tissue so paraffin sections can be sectioned. Decalcification is carried out between fixation and processing steps. What is Decalcification?