Resulting sequencing libraries reveal remarkably abundant (~80% of small RNAs) ~28-34nt tRNA fragments (tRFs), predominantly derived from the 5’ ends of tRNAs (Fig.1A-D, Tables S1 - S2) (6). 5’ tRFs are also abundant in cauda sperm from the bull B. taurus (Table S3), suggesting that tRNA cleavage in gametes is conserved among mammals, and perhap...

2015年12月31日 · Protein restriction in mice affects small RNA (sRNA) levels in mature sperm, with decreased let-7 levels and increased amounts of 5′ fragments of glycine transfer RNAs (tRNAs). In testicular sperm, tRNA fragments are scarce but increase in abundance as sperm mature in the epididymis.

2021年3月3日 · tRNA-derived fragments (tRFs) originated from the specific cleavage of tRNAs and regulate gene expression when the cells are in stressful circumstances. We described that tRF GlnCTG was highly expressed in the balloon-injured rat carotid artery. tRF GlnCTG was found to positively regulate VSMC proliferation via the FAS gene.

转运RNA (transfer RNA, tRNA) 衍生的RNA 片段(transfer RNA-derived fragments, tRFs)是一种新型的小非编码RNA (non-coding RNA, ncRNA)。 tRFs 广泛表达于生物体的各种细胞内。 研究发现tRFs可以通过调控翻译、基因表达和细胞周期对肿瘤的发生发展产生非常重要的作用,其在功能上与微小RNA (microRNA, miRNA) 具有相似之处,但其具体机制尚不完全清楚,还需进一步探究。 本文对tRFs的分类、生物学功能、其异常表达对不同肿瘤的影响以及在肿瘤新型诊断生物标记物和潜 …

tRFs（tRNA-Derived Fragments）是一类长度<40nt，来源于tRNA转录本的非编码小片段RNA。 最近的研究表明，这些小RNA片段具有特定的生物学功能，如抑制基因表达、调节细胞凋亡和跨代表观遗传。 通过对小RNA片段进行高通量测序和分析可鉴定tRFs，并且它们可对应到已知的tRNA基因。 根据tRFs在初级或成熟tRNA转录本上的映射位置，可将其分为不同的类型： tiRNA (tiR)或tRNA halves： 由应激 (和饥饿)诱导的tRNA片段，通过在成熟tRNAs的反密码子环中特 …

tRNA-derived RNA fragments (tRFs) are 18–26 nucleotide small RNAs that are not random degradation products, but are rather specifically cleaved from mature tRNA transcripts. Abundant in stressed or viral-infected cells, the function and potential targets of tRFs are not known.

In the past 2 decades, the discovery of a new class of small RNAs, known as tRNA-derived fragments (tRFs), shed light on a new layer of regulation implicated in many biological processes. tRFs originate from mature tRNAs and are classified according to the tRNA regions that they derive from, namely 3′tRF, 5′tRF, and tRF-halves.

2022年3月24日 · Traditionally, transfer RNAs (tRNAs) specifically decoded messenger RNA (mRNA) and participated in protein translation. tRNA-derived fragments (tRFs), also known as tRNA-derived small RNAs (tsRNAs), are generated by the specific cleavage of pre- and mature tRNAs and are a class of newly defined functional small non-coding RNAs (sncRNAs).

2021年1月1日 · tRNA-derived fragments (tRFs) are a new category of regulatory noncoding RNAs with distinct biological functions in cancers and stress-induced diseases. Herein, we first summarize the classification and biogenesis of tRFs. tRFs are …

Recent studies have revealed that tRNAs are not always the terminal molecules and small RNA fragments can be mapped to precursor tRNA sequences or mature tRNA sequences. tRNA-derived fragments (tRFs) are a novel class of small RNAs in miRNA-size found in a diverse range of organisms and can be the source of small regulatory RNAs, a previously un...