2013年10月5日 · Dihydroethidium (DHE), was used to measure O 2 ‾ in HUVEC and HMVEC following 24 hours of treatment with high glucose. At the end of the treatment period, cells were washed and incubated with 5 μM DHE in phenol red-free treatment medium for 1 hour.

In this study, we utilized human umbilical vein endothelial cells (HUVEC) to examine the roles of ADMA cellular transport and the uncoupling of endothelial nitric oxide synthase (eNOS) in ...

2012年6月18日 · In this study, we utilized human umbilical vein endothelial cells (HUVEC) to examine the roles of ADMA cellular transport and the uncoupling of endothelial nitric oxide synthase (eNOS) in contributing to this phenomenon. Dihydroethidium (DHE) fluorescence was used as an index of oxidative stress.

2013年6月3日 · APS suppresses TNFα-induced adhesion molecule expression by blocking NF-κB signaling and inhibiting ROS generation in HUVECs. The results suggest that APS may be used to treat and prevent...

2022年11月1日 · Herein, we showed that EFs induced human umbilical vein endothelial cells (HUVEC) migration toward the cathode with enhanced autophagy. Genetic ablation of autophagy by silencing the autophagy-related gene (Atg) 5 abolished the EFs-directed migration of HUVEC, indicating that autophagy is definitely required for EFs-guided migration of cells.

由于易于获取和纯度高，人脐静脉内皮细胞（HUVECs）是研究内皮细胞生理学、血管生成和药物发现的最受欢迎的内皮细胞。 HUVECs具有“鹅卵石”形态，细胞生长为单层紧密相对的多边形大细胞，含有原位内皮细胞特征的细胞质包涵体（Weibel-Palade小体）以及大量的平滑肌肌动蛋白，vWF/Factor VIII和CD-31特异性抗体免疫荧光染色呈阳性，并具有摄取乙酰化低密度脂蛋白的能力。 培养的HUVECs也含有与组织供者血型相适应的ABH抗原，但在培养的平滑肌细胞或成 …

HUVEC ,探究五味子木脂素是否靶向Nrf-2 缓解Ang II诱导氧化应激损伤。结果 Sch B(50 μmol·L−1)和Sch C(26 μmol·L−1) 作用于Ang II诱导的HUVEC 损伤模型中,发现Sch B、Sch C 可通过调控抗氧化轴Nrf-2/Keap-1,抑制细胞凋亡(P<0.01 或P<0.001) 和ROS 水平(P<0.05) ,促进HO-1(P<0.05)�. NQO-1(P<0.05)等抗氧化酶的表达,并有效缓解H2O2 诱导的氧化应激损伤。在特异性沉默Nrf-2 的HUVEC 中,发现Sch B、Sch C 可靶向Nrf-2�.

Furthermore, HUVEC coincubated with high vitamin E concentration (150 nM) and catalase inhibitor had higher DCHFDA staining but similar DHE staining, whereas cells incubated with the SOD...

2018年11月24日 · Dihydroethidium (DHE)-derived fluorescence probe detection at 570 nm showed that human umbilical vein endothelial cells (HUVECs) in the model group, treated with hydrogen peroxide (H 2 O 2) produced increased levels of reactive oxygen species (ROS), which were significantly increased compared with the untreated group. However, the ROS content ...

人脐静脉内皮细胞（英Human Umbilical Vein Endothelial Cells，HUVEC）在进行 血管内皮细胞 实验时，通常选用的 细胞模型 为人 脐静脉内皮细胞 （Human umbilical vein endothelial cells，简称HUVECs）。而不是直接采用静脉血管内皮细胞或动脉血管内皮细胞。