Double Holliday junctions (dHJS) are important intermediates of homologous recombination. The separate junctions can each be cleaved by DNA structure-selective endonucleases known as Holliday junction resolvases. Alternatively, double Holliday junctions can be processed by a reaction known as “double Holliday junction dissolution.”

2025年1月1日 · Holliday junction (HJ) resolution in somatic cells occurs in an unbiased manner to produce both crossovers and non-crossovers, suggesting that meiotic dHJ resolution involves unique features to impose bias. Studies involving fine-scale mapping of meiotic crossover events have shown that HJs move across DNA.

2024年2月15日 · dHJs can be removed by two genetically and biochemically distinct pathways, termed dissolution and resolution. dHJ dissolution is carried out by the Bloom syndrome helicase BLM in complex with topoisomerase IIIα (TOP3A) and two structurally related subunits, RMI1 and RMI2 (the BTR complex). 6 BLM helicase activity promotes convergent branch ...

2020年8月19日 · We propose a model for crossover-specific dHJ resolution in which orientation-specific loading of PCNA, during the DNA synthesis that accompanies dHJ formation, helps to direct the MutLγ–MutSγ...

现有的体外实验结果显示：人体细胞中的RecQ家族解旋酶BLM和拓扑异构酶Ⅲα（TopoⅢα）可催化四链中间体包含的两个Holliday连接体（dHJ）的汇集（即相向的分支迁移），并导致非交换产物的形成（该过程被称为“dHJ dissolution”）（121）。

2017年1月3日 · Using RecA-generated α-structures that contained single HJs and a plasmid-based substrate that contains two HJs separated by 165 bp, we found that GEN1 promoted the efficient resolution of both single-HJ and dHJ structures.

2024年9月14日 · Here we show that SC promotes crossover-specific resolution by protecting dHJs from unscheduled and aberrant resolution. When key SC components are conditionally inactivated during pachytene, dHJs are resolved into noncrossover products by Sgs1-Top3-Rmi1 (STR), the yeast ortholog of the human BLM complex 7 .

2014年2月9日 · Repair of DNA double-strand breaks via homologous recombination can produce double Holliday junctions (dHJs) that require enzymatic separation. Topoisomerase IIIα (TopIIIα) together with RMI1...

A group of ubiquitous and highly specialized structure-selective endonucleases catalyze the cleavage of HJs into two disconnected DNA duplexes in a reaction called HJ resolution. These enzymes, called HJ resolvases, have been identified in bacteria and their bacteriophages, archaea, and eukaryotes.

The Double Holliday junction (DHJ) dissolution is promoted by the BLM helicase, the TopoIIIα topoisomerase and the RMI1, which influencing the dynamics of TopIIIα (Bocquet et...