变性高效液相色谱 （denaturing high performance liquid chromatography，DHPLC）是一项在单链构象多态性 (SSCP)和变性梯度凝胶电泳 (DGGE)基础上发展起来的新的杂合双链突变检测技术，可自动检测单碱基替代及小片段核苷酸的插入或缺失。

DHPLC是denaturing high performance liquid chromatography的简称，意思是变性高效液相色谱分析。 在部分变性的条件下，通过杂合与纯合二倍体在柱中保留时间的差异，发现 DNA突变。 异源双链DNA与同源双链 DNA 的解链特性不同，在部分变性条件下，异源双链因有错配区的存在而更易变性，在色谱柱中的保留时间短于同源双链，故先被洗脱下来，在色谱图中表现为双峰或多峰的洗脱曲线。 近年来建立并迅速发展的DHPLC是一种新型基因突变筛查技术，既能够自动化、 …

Denaturing High Performance Liquid Chromatography (DHPLC) is a method of chromatography for the detection of base substitutions, small deletions or insertions in the DNA. [1] Due to its speed and high resolution, this method is particularly useful for finding polymorphisms in DNA.

Denaturing high-performance liquid chromatography (DHPLC) compares two or more chromosomes as a mixture of denatured and reannealed PCR amplicons, revealing the presence of a mutation by the differential retention of homo- and heteroduplex DNA on reversed-phase chromatography supports under partial …

变性高效液相色谱(dhplc)是一种高通量筛选dna序列变异的新技术,从该仪器设备的组成、工作原理、基本操作方法、主要技术特点等作一综述,并对其在基因组领域的应用如snp分析、双链dna片段分析、微卫星分析、mrna定量分析、引物纯度检测等方面及在医学、遗传学 ...

Denaturing high-performance liquid chromatography (DHPLC) is a method that detects polymorphisms using the differential elution time of DNA homoduplexes and heteroduplexes (where a SNP is present) from a liquid chromatography column 30.

2010年3月23日 · dhplc技术也称为wave核苷酸片段分析系统。 利用高效液相色谱原理，PCR扩增后的DNA片段与缓冲液（TEAA）混合形成液相，流动相被高压驱动，通过一个DNA分离柱——DNA Sep柱，该柱可对DNA片段进行分离和分析。

变性高效液相色谱(dhplc)技术是一种能用于快速，自动和高通量检测基因的技术平台。在分子生物学研究和临床基因诊断领域中，dhplc技术可应用于dna的绝大部分检测，在很多领域已建立了快速、自动化核酸分析新方法和新标准。

2001年12月1日 · Denaturing high pressure liquid chromatography (DHPLC) is a relatively new technique, which uses heteroduplex formation between wild-type and mutated DNA strands...

Denaturing high-performance liquid chromatography (DHPLC) compares two or more chromo-somes as a mixture of denatured and reannealed PCR amplicons, revealing the presence of a mutation by the differential retention of homo- and heteroduplex DNA on reversed-phase chro-matography supports under partial denaturation.