Differently, here we describe DNA damage In situ ligation followed by Proximity Ligation Assay (DI-PLA), that allows sensitive detection of physical DSBs in fixed cells, through direct labeling of the DSBs with biotinylated oligonucleotides, and subsequent signal amplification by PLA between biotin and a partner protein in the proximity of the ...

2018年11月25日 · Here we describe a recently published method, DNA damage In situ ligation followed by proximity ligation assay (DI-PLA), to detect physical DSBs in proximity of a target protein in individual cells (Fig. 1). Briefly, adherent cells are grown on glass coverslips, fixed, and permeabilized to allow enzymatic modification of genomic DNA.

Duolink ® Proximity Ligation Assays (PLA) enable the studying of endogenous Protein-Protein Interactions (PPI) and Post-Translational Modifications, providing a fluorescent signal for a single event that can be visualized by either microscopy or flow cytometry.

Here, we developed a method, named 'DNA damage in situ ligation followed by proximity ligation assay' (DI-PLA) for the detection and imaging of DSBs in cells. DI-PLA is based on the capture of free DNA ends in fixed cells in situ, by ligation to biotinylated double-stranded DNA oligonucleotides, which are next recognized by antibiotin anti-bodies.

Here, we propose a novel method, named DI‐PLA, to visualize DNA DSBs at a single‐cell level, which, through the direct tagging of DNA ends, reliably detects only unrepaired DSBs in close physical proximity with an activated DDR protein. By DI‐PLA, we were able to detect DSBs generated by several sources in both cultured cells, and tissues.

DI-PLA and STRIDE share similarities in employing a proximity ligation assay (PLA) reaction to produce fluorescence, whereas STRIDE and TUDEL share similarity in utilizing TdT to incorporate the oligonucleotide analogues 5-bromo-2′-deoxyuridine (BrdU) and …

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2019年1月1日 · Differently, here we describe DNA damage In situ ligation followed by Proximity Ligation Assay (DI-PLA), that allows sensitive detection of physical DSBs in fixed cells, through direct labeling...

Correction to: DNA Damage In situ Ligation followed by Proximity Ligation Assay (DI-PLA) Methods Mol Biol. 2019:1896:C3. doi: 10.1007/978-1-4939-8931-7_20. Authors Alessandro Galbiati 1 , Fabrizio d'Adda di Fagagna 2 3 Affiliations 1 Oncology IMED ...

What does DI-PLA abbreviation stand for? Explore the list of 1 best DI-PLA meaning form based on popularity. Most common DI-PLA abbreviation full form updated in May 2020.