A phosphodiesterase (PDE) is an enzyme that breaks a phosphodiester bond. Usually, phosphodiesterase refers to cyclic nucleotide phosphodiesterases, which have great clinical significance and are described below.

磷酸二酯酶 (PDEs) 是一系列水解 cAMP 及 cGMP 的水解酶，是调节 cAMP 和 cGMP 水平的重要环节，针对 PDEs 尤其是 PDE3 和 PDE5 的干预对于 心力衰竭 治疗有着重要意义. 环磷酸腺苷 (cAMP)与环磷酸鸟苷 (cGMP)是细胞内重要的第二信使，心肌细胞的活动也与他们密切相关. cAMP 由 腺苷酸环化酶 催化三磷酸腺苷形成，其降解由不同磷酸二酯酶 (PDEs)催化PDEs 是能够催化水解环磷酸核苷磷酸二酯键. cGMP 由 鸟苷酸环化酶 在心肌细胞中的主要作用似乎与 cAMP 相 …

PDEs是一个 多基因 的大家族，它包括11型共30余种具有不同 底物 专一性、 酶动力学 特征、调控特点以及细胞与亚细胞分布区域不同的磷酸二酯酶同功酶 PDEs拥有相似的结构，均包含调控和催化两个功能区域。 各型PDEs催化区的氨基酸序列75% 以上相同．显示出家族成员间的同源性．并决定着对底物或 抑制剂 的专一性。 PDEs具有不同的底物专一性：PDE 4、7、8专一作用于cAMP，而PDE 5、6、9则选择性地作用于cGMP。 PDE3以相似的亲和力与cAMP和cGMP结 …

1991年11月11日 · We focused on 3'-repair diesterases, which process DNA ends at oxidative strand breaks by removing 3'-blocking fragments of deoxyribose that prevent DNA repair synthesis. Two enzymes were found in a variety of mouse, bovine and human tissues and cultured cells. The two activities were purified to differing degrees from HeLa cells.

1988年12月5日 · Yeast DNA 3′-repair diesterase is the major cellular apurinic/apyrimidinic endonuclease: substrate specificity and kinetics. DNA strand breaks with damaged 3′ termini are potentially toxic lesions caused by free radicals.

Phosphodiesterases are enzymes that catalyze the hydrolysis of the 3′ cyclic phosphate bond of cyclic nucleotides. To date, 11 PDE gene families have been identified, based on their amino acid sequences, biochemical properties, and inhibitor profiles.

PDE1 is a dual substrate (cAMP and cGMP) diesterase and a promising target for the treatment of heart disease, cancer, neurological disorder and other endocrine abnormalities.

To optimize diesterase activity on the AP scaffold, binding interactions with the diester functionality on the transferred phosphoryl group (R' in Scheme 1B) could be introduced to assist with specific binding and positioning of diester substrates.

Enzymes cleaving bonds in phosphodiesters, such as those in cAMP or between nucleotides in nucleic acids, liberating smaller polynucleotide or oligonucleotide units or mononucleotides but not orthophosphate. Synonym (s): phosphodiester hydrolases.

2007年7月17日 · We report that the PAPs from pig and from red kidney bean efficiently hydrolyze the diesters methyl nitrophenyl phosphate and ethyl nitrophenyl phosphate. The details of the diester reaction give insights into the sequential steps involved in …