Bright and stable monomeric green fluorescent protein derived
2024年2月26日 · The tandem dimeric version of StayGold, td8ox2StayGold, outperformed all tested GFPs in terms of brightness and photostability in live HEK cells and given its good …
TurboGFP: Properties, Sequence, MW, origin | Proteintech Group
TurboGFP is a bright dimeric green fluorescent protein derived from CopGFP of the copepod Pontellina plumata. CopGFP has been first published in 2004 (Shagin et al., 2004) Origin of …
ZEISS Microscopy Online Campus | Introduction to Fluorescent …
For many of the Aequorea GFP variants, dimerization can be either significantly reduced or eliminated completely by replacing the hydrophobic amino acid side chains in the dimer …
A new bright green-emitting fluorescent protein: Engineered …
We present two proteins, mVFP and mVFP1, that are both exclusively monomeric, and one, dVFP, which is dimeric. We characterized the spectroscopic properties of VFP and its variants …
A highly photostable and bright green fluorescent protein
2022年4月25日 · Here we present StayGold, a green fluorescent protein (GFP) derived from the jellyfish Cytaeis uchidae. StayGold is over one order of magnitude more photostable than any …
Identification and Characterisation of a pH-stable GFP
2016年6月21日 · We report the engineering and characterisation of tandem dimer GFP (pH-tdGFP), a bright and stable GFP that can be efficiently excited and maintains its fluorescence …
Combined FCS and PCH Analysis to Quantify Protein Dimerization …
2021年7月7日 · Here we present a methodology to measure the extent of dimerization of GFP-tagged proteins in living cells, using a combination of fluorescence correlation spectroscopy …
Cation Specific Effects on the Domain-Domain Interaction of ...
2020年7月9日 · Specific monovalent cation effects on the domain-domain interaction of heterogeneous dimeric protein were investigated using green fluorescent protein (GFP) …
A photostable monomeric superfolder green fluorescent protein
The green fluorescent protein (GFP) from Aequorea victoria has been engineered extensively in the past to generate variants suitable for protein tagging. Early efforts produced the enhanced …
A genetically encoded toolkit of functionalized nanobodies against ...
2019年5月23日 · We developed two methods to allow crosslinking of RFP-tagged and GFP-tagged proteins: a dimeric nanobody, and co-expression of RFP-targeting and GFP-targeting …