2013年4月4日 · Our data demonstrate that DnaC opens DnaB by a mechanism akin to that of polymerase clamp loaders, and indicate that bacterial replicative helicases, like their …

2024年5月1日 · In this study, we took a biochemical approach to examine the impact of dnaC mutations in five DnaC variants: DnaC 809 (E176G), DnaC 809,820 (E176G, K178N), DnaC …

1989年2月15日 · The dnaC protein of Escherichia coli, by forming a complex with the dnaB protein, facilitates the interactions with single-stranded DNA that enable dnaB to perform its …

2017年12月12日 · Former studies relying on hydrogen/deuterium exchange analysis suggest that DnaC bound to DnaB alters the conformation of the N-terminal domain (NTD) of DnaB to …

2010年1月1日 · An AAA+ ATPase, DnaC delivers DnaB helicase at the E. coli chromosomal origin by a poorly understood process. This report shows that mutant proteins bearing alanine …

We propose a probable structure for the DnaA–DnaB–DnaC complex, which could be relevant to the process of DnaB loading onto oriC. Loading of replicative helicases onto single-stranded …

2016年1月8日 · We used the method of hydrogen/deuterium exchange mass spectrometry to address the importance of DnaB-DnaC complex formation as a prerequisite for helicase …

We present the 26 Å resolution three-dimensional structure of the DnaB hexamer in complex with its loading partner, DnaC, obtained from cryo-electron microscopy.

2013年4月11日 · Dedicated AAA+ ATPases deposit hexameric ring-shaped helicases onto DNA to promote replication in cellular organisms. To understand how loading occurs, we used …

questions. Why does DnaC require ATP to deliver DnaB to oriC, and what induces the dissociation of DnaC from DnaB? Of the AAA+ motifs of DnaC, the box VII motif contains two …