DnaG is a bacterial DNA primase and is encoded by the dnaG gene. The enzyme DnaG, and any other DNA primase, synthesizes short strands of RNA known as oligonucleotides during DNA replication. These oligonucleotides are known as primers because they act as a starting point for DNA synthesis.

DnaG is a primase which synthesizes a short RNA to prime DNA chain elongation catalyzed by pol III HE. From: Encyclopedia of Biological Chemistry (Third Edition), 2021

2017年4月6日 · To better understand the structure and function of primase and address the interactions between DnaG and specific DNA template, we have carried out structural and functional analysis on DnaG...

2007年10月19日 · DnaG increases both the NTPase and the helicase activities of DnaB (9), and DnaB both increases and modulates the synthesis of RNA primers by DnaG (1). DnaG consists of three domains, an N-terminal zinc-binding domain (ZBD), an RNA polymerase domain (RPD), and a C-terminal HBD.

Compounds that inhibit bacterial DnaG primase have been developed using different approaches. In this paper, we provide an overview of the current literature on DNA primases as novel drug targets and the methods used to find their inhibitors.

Mycobacteria contain genes for several DNA-dependent RNA primases, including dnaG, which encodes an essential replication enzyme that has been proposed as a target for antituberculosis compounds. An in silico analysis revealed that mycobacteria also possess archaeo-eukaryotic superfamily primases (AEPs) of unknown function.

Conversion of the viral DNA of phage G4 to the duplex form provided an opportunity to isolate and determine the function of the dnaG protein, the product of a gene known to be essential for replication of the Escherichia coli chromosome.

DnaG is the replicative primase that synthesizes RNA primers for extension by DNA polymerases. The E. coli primase transcribes roughly 2000 RNA primers per replication cycle. 12 The process consists of five steps: template binding, NTP binding, initiation, extension of the primer, and transfer of the primer to DNA polymerase III.

DnaG is a bacterial DNA primase and is encoded by the dnaG gene. The enzyme DnaG, and any other DNA primase, synthesizes short strands of RNA known as oligonucleotides during DNA replication. These oligonucleotides are known as primers because they act as a starting point for DNA synthesis.

The DnaB protein is a major replicative DNA helicase which moves along the lagging strand in the 5′→3′ direction and opens up the duplex DNA at the tip of the replication fork to expose a pair of single-stranded DNA templates. The DnaG protein is a primase which synthesizes a short RNA to prime DNA chain elongation catalyzed by Pol III HE.