DpnI cleaves only when its recognition site is methylated. Cleavage of mammalian genomic DNA is blocked by overlapping CpG methylation. Need a custom/large volume order? Contact us. An E. coli strain that carries the DpnI gene from Diplococcus pneumoniae G41 (S. Lacks).

此外，NEB 提供的星选酶 DpnI 还有以下特性： 省时酶：能在 5-15 分钟内消化底物 DNA，也可过夜酶切而不会降解 DNA; 在兼容性强的 rCutSmart Buffer 中 100% 活性，便于双酶切; 随酶送 6X 紫色上样染料（NEB #B7024），无紫外阴影; DpnI：用于点突变实验中模板去除

100 units of DpnI incubated for 16 hours at 37ºC results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis. Protein Purity Assay (SDS-PAGE) - DpnI is >95% pure as determined by SDS PAGE analysis using Coomassie Blue detection. Product Name: DpnI Catalog #: R0176S/L Concentration: 20,000 units/ml

大肠杆菌菌株，携带有克隆自肺炎双球菌（Diplococcus pneumoniae）G41（S. Lacks）的 DpnI 基因。 一个单位是指在 50 µl 的总反应体系中，37℃ 条件下，1 小时内酶切 1 µg pBR322 DNA（dam 甲基化）所需的酶量。 只有当识别位点被甲基化时，DpnI 才能酶切。 从 dam + 菌株纯化而来的 DNA 才是 DpnI 的酶切底物。 CpG 甲基化与酶切位点重叠阻断酶切。 Why Choose Recombinant Enzymes? Will DpnI cleave hemimethylated DNA? What's the difference …

当必须在PCR反应中使用较多的质粒模板时，建议用DpnI（NEB）对PCR产物进行消化。 DpnI只裂解来源于大肠杆菌甲基化后的质粒DNA（模板），但不裂解PCR产物，因为PCR产物没有被甲基化。 DpnI消化方案： 1. 在总共10微升的反应中，将5-8微升的PCR产物与1微升的10X缓冲液 ...

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DpnII and Sau3AI are isoschizomers of MboI. Cleaves to leave a 5´ GATC extension which can be efficiently ligated to DNA fragments generated by BamHI, BclI, BglII, MboI, Sau3AI and BstYI. This enzyme is blocked by dam methylation. More information can be found at Dam-Dcm and CpG Methylation. Will exhibit star activity in NEBuffer r3.1.

该酶可在特定位点切开DNA，产生可体外连接的DNA片段。

100 units of DpnI incubated for 16 hours at 37 oC results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis. DpnI is >95% pure as determined by SDS PAGE analysis using Coomassie Blue detection.

100 units of DpnI incubated for 16 hours at 37ºC results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis. Protein Purity Assay (SDS-PAGE) - DpnI is >95% pure as determined by SDS PAGE analysis using …