Recent advances on halohydrin dehalogenases—from enzyme …
2016年8月8日 · These enzymes, also called halohydrin dehalogenases (HHDHs), haloalcohol dehalogenases, or halohydrin hydrogen-halide-lyases, belong to the enzyme class of lyases …
A high-throughput screening assay for the directed evolution …
2020年3月10日 · To use directed evolution to unleash the latent potential of HHDHs for the synthesis of β-substituted alcohols, we report a high-throughput assay for screening HHDHs …
Intact-cell detection of halohydrin dehalogenase activity and ...
2024年11月1日 · Halohydrin dehalogenase (HHDH) can degrade 1,2,3-trichloropropanol and other halogenated alcohols. These enzymes exhibit stereoselectivity and can be used to …
HheG，一种对环氧化物具有活性的卤代醇脱卤酶,ACS Catalysis - X …
2017年9月13日 · 卤代醇脱卤化酶（HHDH）具有混杂的环氧化物开环活性和一组带负电荷的亲核试剂，因此具有生物技术意义，能够形成C–C，C–N或C–O键。 HHDH特异性序列基序的最 …
Properties and biotechnological applications of halohydrin ...
2012年11月1日 · Halohydrin dehalogenases (HHDHs) are lyases that catalyze the cleavage of carbon–halogen bond of halohydrins. They also can catalyze the reverse reaction in the …
卤代醇脱卤酶的合理设计在水-有机两相体系中高效生产高活性和对 …
摘要 卤代醇脱卤酶（HHDHs）是生产有价值的手性环氧化物和β-取代醇的重要生物催化剂。 来自放射杆菌 (HheC) 的 HHDH 显示出 (S)-表氯醇 ( (S)-ECH) 生物合成的巨大潜力。 然而，包括 …
Rational design of halohydrin dehalogenase for efficient chiral ...
2020年9月15日 · Halohydrin dehalogenases (HHDHs) represent as important biocatalysts for the production of valuable chiral epoxides and β-substituted alcohols. The HHDH from A. …
磁性生物炭中的卤代醇脱卤酶固定化可持续卤代烃生物降解和生物 …
关于卤代醇脱卤酶 (HHDH) 在生物转化和生物降解方面显示出巨大潜力的事实，开发适用于这些应用的固定化 HHDH 将非常重要。 在此，制备了功能化磁性生物炭，用于高效的酶固定化、易 …
An efficient high-throughput screening assay for rapid directed ...
Here, a novel high-throughput screening (HTS) methodology for directed evolution of HHDH was developed based on the colorimetric determination of azide. In this method, azide was …
Halohydrin dehalogenase (HHDH) distinct structural elements and …
Halohydrin dehalogenases (HHDH) are industrially relevant biocatalysts exhibiting a promiscuous epoxide-ring opening reactivity in the presence of small nucleophiles, thus...
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