差示扫描荧光法（Differential Scanning Fluorimetry, 简称DSF），是在荧光定量PCR仪上缓慢加热样品，在加热的过程中检测荧光染料与结构发生改变的蛋白质相结合的量，来评价蛋白质热稳定性的方法。

Differential Scanning Fluorimetry (DSF) measures protein unfolding by monitory changes in fluorescence as a function of temperature. Conventional DSF uses a hydrophobic fluorescent dye that binds to proteins as they unfold.

The most common method for measuring protein thermal shifts is differential scanning fluorimetry (DSF). DSF methodology includes techniques such as nanoDSF, [1][2] which relies on the intrinsic fluorescence from native tryptophan or tyrosine residues, and Thermofluor, which utilizes extrinsic fluorogenic dyes. [3]

2023年12月15日 · Differential scanning fluorimetry (DSF) is a widely used technique for determining the apparent melting temperature (Tma) of a purified protein. Here, we present a protocol for performing and optimizing DSF experiments. We describe steps for designing and performing the experiment, analyzing data, and optimization.

2022年9月20日 · 差示扫描荧光法（DSF），也被称为thermal shift assay（TSA），是表征蛋白热稳定性的常用方法之一，广泛应用于蛋白配体互作表征，突变体、缓冲液、去垢剂筛选等领域。

2024年5月14日 · Differential scanning fluorimetry (DSF) is a technique that reports protein thermal stability via the selective recognition of unfolded states by...

2020年1月31日 · DSF is a cost-effective, parallelizable, practical, and accessible biophysical technique widely used as a method to track both protein folding state and thermal stability. It provides a reliable tool to examine protein unfolding by slowly heating it …

2022年3月4日 · 差示扫描荧光法 (differential scanning fluorimetry, DSF) 是一种方便快捷的高通量药物筛选及靶标发现的方法，该方法具有蛋白样品损耗少、通量高、温度变化范围广及数据准确等优点，被广泛用于蛋白质稳定性（蛋白质热稳定性参数及其影响因素）、蛋白结构和构象、蛋白-配体相互作用及蛋白质稳定剂、抑制剂、辅助因子等领域的研究。 差示扫描荧光法（DSF）以荧光染料为指示剂，检测蛋白的热稳定性。 目前最常用的染料是 SYPRO Orange，SYPRO Orange …

Differential scanning fluorimetry (DSF) is a widely used technique for determining the apparent melting temperature (Tma) of a purified protein. Here, we present a protocol for performing and optimizing DSF experiments. We describe steps for designing and performing the experiment, analyzing data, and optimization.

2020年12月4日 · Differential scanning fluorimetry (DSF) can be used to detect the binding of a small molecule ligand to a purified target protein. Upon binding with certain ligands, the protein can be stabilized from thermal denaturation. DSF uses a fluorescent dye and Real-Time PCR instrument to detect the unfolding process of proteins during thermal ...