2024年10月7日 · Our findings reveal that DSF ameliorates osteoporotic changes induced by ovariectomy (OVX) via the suppression of osteoclast differentiation without affecting osteoblast functions. We focused on a target of OVX, FROUNT, and its binding receptors CCR2 and CCR5, in osteoclast precursor cells (OCPs).

Dithiocarbamates (DTC), a sulfhydryl group containing compounds, are extensively used by humans that include metam and thiram due to their pesticide properties, and disulfiram (DSF) as an alcohol deterrent. We screened these DTC in an osteoblast viability assay. DSF exhibited the highest cytotoxicit …

2015年4月30日 · DSF treatment dose-dependently inhibited both mouse and human osteoclastogenesis, especially at early stages of differentiation. This inhibition correlated with a decrease in the expression of key osteoclastic marker genes including CtsK, TRAP, DC-STAMP and Atp6v0d2 as well as a reduction in bone resorption in vitro.

Results: DSF administration ameliorated ovariectomy-induced bone loss and mitigated the increase of osteoclasts without affecting osteoblastogenesis. The scRNA-seq data revealed that osteoclast precursor cells expressed Nup85, Ccr2, and Ccr5.

2015年4月30日 · Considering that MAPK, NF-κB pathways and V-ATPases all play crucial roles at various stages of the OC formation-bone resorption cycle, DSF represents an attractive antiresorptive candidate. Here, we have examined the antiresorptive potential and molecular action of DSF on OC formation, bone resorption and acidification.

2015年4月30日 · DSF treatment dose-dependently inhibited both mouse and human osteoclastogenesis, especially at early stages of differentiation. This inhibition correlated with a decrease in the expression of key osteoclastic marker genes including CtsK, TRAP, DC-STAMP and Atp6v0d2 as well as a reduction in bone resorption in vitro.

2024年1月24日 · Knockout of RNF115 or treatment with DSF significantly inhibit systemic inflammation and autoimmune lethality and restore immune cell development in Trex1–/– mice and STINGN153S/WT bone marrow...

DSF has a cytotoxic effect on bone marrow monocytes and dosedependently reduced mouse and human osteoclastogenesis at an early stage of differentiation likely via the induction of...

2024年3月12日 · This study aimed to explore the effect of enhancing the interfragmentary compression force (IFCF) on fixation stability in lateral TPFs in normal and osteoporotic bones using finite element analysis. Finite element models of DSF in normal bone and LPF in normal and osteoporotic bones were established to simulate the fixations of lateral TPF.

Bone discs were fixed and assessed for bone resorption by toluidine blue staining (resorption pits shown by yellow arrow heads). The total resorbed area on each bone disc was quantified as a percentage of the total bone disc area (mean ± SD; **: P