Learn about the complementary in situ hybridization and immunohistochemistry technique, called dual ISH-IHC, and specifically the novel RNAscope technology and its benefits.

RNA in situ hybridization (ISH) and immunohistochemistry (IHC) are well-established methods providing unique RNA and protein expression quantification in a morphological context. Often considered complementary technologies, they are bridging …

However, there is a paucity of techniques for dual immunohistochemical (IHC) and mRNA in situ hybridization (ISH) detection. The recent development of improved non-radioactive detection systems and simplified ISH protocols has prompted us to develop a tyramide signal amplification method for sequential multi-label fluorescent ISH and IHC ...

Read our Protocol for Dual In Situ Hybridization and Immunohistochemistry Application Using ACD’s RNAScope®. Enjoy Tips, Techniques and Troubleshooting.

IHC双重酶底物染色检测系统使用免疫酶检测法对组织切片上的两种抗原进行染色。 通过本文了解我们的双重染色IHC试剂盒的应用，为您的多色IHC实验提供最佳的实验方案。

2024年6月24日 · We developed a dual ISH-IHC protocol because fundamentally, we want to be able to see where the mRNA is accumulating. We want to identify which tissue and which cell type is producing it. That’s why RNAscope™ ISH is really important in …

Introducing our protease-free pretreatment workflow for sequential RNA-Protein detection. Analysis of both RNA and protein provides invaluable information for understanding the regulation of expression. Both ISH and IHC technologies share common features that make them unique amongst the protein and RNA detection technologies.

2024年1月23日 · Procedure for ImmunoEnzyme Double Staining. Note: prior to perform double labeling, it is important to test each primary antibody individually and select the best pretreatment (s) for each antibody. It will be ideal if the two primary antibodies require same pretreatment.

Read our Protocol for Dual In Situ Hybridization and Immunohistochemistry Application Using ACD’s RNAScope®. Enjoy Tips, Techniques and Troubleshooting.

2019年12月13日 · We propose RNAscope dual ISH–IHC as an extremely powerful method to study angiogenesis in DLBCLs, because it allows one to answer important biological questions concerning angiogenesis mechanisms and endothelium features that are difficult to address using other single methods.