2003年6月19日 · Several characteristic features of E46Q such as an alkali shift in the pK(a) and the rapid photocycle can be explained by this weakened hydrogen bond. Furthermore, the red shift in the absorption maximum in E46Q can be explained by the delocalization of the electron on the phenolic oxygen of p-coumaric acid owing to the weakening of this ...

2017年9月1日 · To elucidate the role of internal proton transfer in the photodynamics of photoactive yellow protein (PYP), the photocycle of the Glu46Gln mutant of PYP (E46Q-PYP) is investigated by transient grating (TG) spectroscopy.

2005年1月1日 · Here, we analyze by singular value decomposition a comprehensive time-resolved crystallographic data set of the E46Q mutant of PYP throughout the photocycle spanning 10 ns–100 ms. We identify and refine the structures of five distinct intermediates and provide a plausible chemical kinetic mechanism for their inter conversion.

Photoactive yellow protein from Ectothiorhodospira halophila is a photoreceptor protein involved in the negative phototaxis of this bacterium. Its chromophore (p-coumaric acid) is deprotonated in the ground state, which is stabilized by a hydrogen …

2017年9月1日 · To elucidate the role of internal proton transfer in the photodynamics of photoactive yellow protein (PYP), the photocycle of the Glu46Gln mutant of PYP (E46Q-PYP) is investigated by transient grating (TG) spectroscopy.

Photoactive yellow protein (PYP), a small (14 kDa) water-soluble protein, isolated from Halorhodospira halophila, is implicated in the blue-light negative-phototactic response of the bacterium (Meyer et al., 1987), thereby protecting it from harmful exposure to …

Similar analysis of density maps of the E46Q mutant of PYP resulted in two time-independent density maps, both of which are structurally homogeneous and correspond to I T (Fig. 3d) and pR 1...

Here, we ana-lyze by singular value decomposition a comprehen-sive time-resolved crystallographic data set of the E46Q mutant of PYP throughout the photocycle span-ning 10 ns–100 ms. We identify and refine the struc-tures of five distinct intermediates and provide a plausible chemical kinetic mechanism for their inter-conversion.

2004年12月1日 · The structure of E46Q mutant protein was determined at 1.2 A resolution, revealing that the phenolic O atom of p-coumaric acid is hydrogen bonded to NH(2) of Gln46 in E46Q with a longer distance (2.86 +/- 0.02 A) than its …

2016年9月16日 · To validate our findings, we carried out neutron crystallographic analysis of the E46Q mutant of PYP. The resultant structure revealed that the proportion of the cationic form is higher in E46Q than in WT, although the cationic and neutral forms of Arg52 coexist in E46Q.