An assay to image neuronal microtubule dynamics in mice
2014年9月12日 · To assay such dynamics by in vivo imaging in the mammalian nervous system, we generated transgenic mice that express EB3 fused to yellow fluorescent protein (YFP) …
Fine structure and dynamics of EB3 binding zones on …
2019年7月31日 · To study fine structure and dynamics of EB-proteins distribution on MTs in live cells, we expressed EB3 tagged with red fluorescent protein (RFP) in 3T3 mouse fibroblasts.
Molecular understanding of label-free second harmonic imaging …
2019年8月6日 · Live recordings of EB3 and SH imaging was followed by immunohistochemistry to identify the axonal and dendritic processes (Fig. 2b).
Employment-Based Immigration: Third Preference EB-3 | USCIS
2025年1月13日 · Third preference petitions are usually accompanied by an a signed Form ETA-9089, Application for Permanent Employment Certification, approved by DOL, or, for labor …
Live imaging of microtubule dynamics at excitatory presynaptic …
2021年3月3日 · Here, we describe a protocol for setting up and analyzing live image recordings of primary neurons and acute hippocampal slices expressing tagged versions of the MT plus end …
EB3 Regulates Microtubule Dynamics at the Cell Cortex and Is …
2007年8月7日 · EB3-depleted cells show disorganized microtubules and fail to stabilize polarized membrane protrusions. Using live-cell imaging, we show that EB3 is necessary for the …
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EB3 imaging to detect microtubule growth following Tubb3 …
EB3 imaging to detect microtubule growth following Tubb3 knockdown. a Live cell imaging of DIV 12 cultured hippocampal neurons cotransfected with EB3-Tomato and control or Tubb3 …
To investigate MT dynamics, we perform live-cell imaging of neurons transfected with a fluorescently tagged version of the microtubule plus-end tracking protein (+TIP) EB3. This …
EB3 regulates microtubule dynamics at the cell cortex and is
2007年8月7日 · EB3-depleted cells show disorganized microtubules and fail to stabilize polarized membrane protrusions. Using live-cell imaging, we show that EB3 is necessary for the …