EGF-responsive interleukin-6 and interleukin-8 were synergistically enhanced by EGF stimulation and mechanical strain. We show here in immortalized and primary BMSC that EGFR signaling enhances mechanotransduction, indicating that the EGF system is a mechanosensitizer in BMSC.

HB-EGF promoted BMSC proliferation via the Akt1 and Erk pathways but inhibited BMSC differentiation via restraining Smad1/5/8 activation. However, Dermo1-HB-EGF mice showed normal osteoclastogenesis and bone resorption.

Our findings demonstrate that cyclic strain and electrical co-stimulation have a synergetic effect on EGF and FGF2-induced rat BMSC neural differentiation by upregulating neural markers and neurotropic mediators and increase calcium influx, intracellular cAMP, and phosphorylation of ERK1/2 and AKT.

HB‐EGF inhibits BMSC in vitro osteogenic and chondrogenic differentiation via Smad1 signaling. To understand the molecular mechanisms by which HB‐EGF regulates BMSC proliferation and differentiation, we compared the activation of HB‐EGF downstream signaling pathways in BMSCs isolated from Dermo1‐HB‐EGF and

2021年5月23日 · egf对msc的作用机制研究是目前所有生长因子中最详尽的一个。 egf受体（egfr/erbb-1）是具有内在酪氨酸激酶活性的原型生长因子受体，广泛表达于多种细胞类型，包括上皮细胞和间充质细胞谱系[13]。

2018年12月14日 · HB-EGF通过Akt1和Erk途径促进BMSC增殖，但通过抑制Smad1 / 5/8激活抑制BMSC分化。 但是，Dermo1-HB-EGF小鼠表现出正常的破骨细胞生成和骨吸收。 这些结果揭示了自分泌或旁分泌HB-EGF在间充质基质细胞增殖和分化中的重要功能，并建议需要严格控制EGF信 …

In this concept paper, we focus on the potential use of epidermal growth factor (EGF), prototypal growth factor for enhancing the harvesting and/or differentiation of MSCs. Soluble EGF was shown to augment MSC proliferation while preserving early progenitors within MSC population, and thus did not induce differentiation.

Bone marrow-derived mesenchymal stem cells (BMSCs) play a critical role in the osseointegration of bone and orthopedic implant. However, osseointegration between the Ti-based implants and the surrounding bone tissue must be improved due to titanium’s inherent defects.

2015年11月26日 · 摘要： 目的 探究胰岛素样生长因子结合蛋白4（IGFBP4）对骨髓间充质干细胞（BMSC）增殖和神经分化的影响。 方法 通过全骨髓贴壁法分离培养BMSCs，免疫荧光检测IGFBP4在P1、P4、P6、P8和P10 BMSCs的表达。 取第4代BMSC细胞，分为单纯培养基组和添加IGFBP4组，用CCK8法检测吸光光度值。 再将BMSC细胞分为3组，诱导培养基NeuroCult培养再分别添加EGF+bFGF和EGF+bFGF+IGFBP4，诱导其向神经祖细胞分化，免疫荧光染色检 …

2021年12月8日 · ALCAM-knockdown myeloma cells displayed enhanced colony formation in the presence of bone marrow stromal cells (BMSCs). BMSCs supported myeloma colony formation by secreted epidermal growth factor (EGF), which bound with its receptor (EGFR) on myeloma cells and activated Mek/Erk cell signaling, PI3K/Akt cell signaling, and hedgehog pathway.