Eosin methylene blue (EMB, also known as " Levine's formulation ") is a selective and differential media used for the identification of Gram-negative bacteria, [1] specifically the Enterobacteriaceae. EMB inhibits the growth of most Gram-positive bacteria. EMB is often used to confirm the presence of coliforms in a sample.

2022年1月4日 · Eosin Methylene Blue (EMB) agar is a differential microbiological medium, which slightly inhibits the growth of Gram-positive bacteria and provides a color indicator distinguishing between organisms that ferment lactose (e.g., E. coli) …

Eosin Methylene Blue (EMB) agar is a selective and differential culture medium. It selectively promotes the growth of Gram-negative bacteria and aids in the differentiation of lactose fermenter and non-lactose fermenting colonies. EMB Agar is used for the isolation of fecal coliforms.

Methylene blue inhibits the gram + bacteria (eosin to a lesser extent), while eosin changes color, to a dark purple, when the medium around the colony becomes acidic. EMB contains lactose and sucrose sugars, but it is the lactose that is the key to the medium.

Eosin-methylene blue (EMB) agar plate inoculated with Escherichia coli (a gram-negative coliform bacterium) showing good growth of dark blue-black colonies with metallic green sheen indicating vigorous fermentation of lactose and acid production …

2022年3月7日 · The eosin methylene blue agar (EMB agar) is a selective staining for gram-negative bacteria. It contains two dyes in the ratio of 6:1 and is both toxic to gram-positive …

2024年3月7日 · Eosin Methylene Blue Agar (EMB agar) is a differential microbiological medium that inhibits Gram-positive bacterial growth and utilizes a color indicator to differentiate between lactose fermenting organisms like E. coli and non-fermenting ones such as …

伊红美蓝培养基（eosin-methylene blue medium,简称EMB medium），一般用于检测大肠杆菌。 伊红为酸性染料，美蓝为碱性染料。 伊红美蓝琼脂培养基在药典中又名曙红亚甲蓝琼脂培养基。

On EMB Agar, Levine, isolated colonies of lactose-fermenting bacteria appear brown to blue-black in color. Escherichia coli appears as large, blue-black colonies, often with a green metallic sheen.

Eosin-methylene blue agar is selective for gram-negative bacteria against gram-positive bacteria. In addition, EMB agar is useful in isolation and differentiation of the various gram-negative bacilli and enteric bacilli, generally known as coliforms and fecal coliforms respectively (8).