Mouse Emd qPCR Primer Pair，即小鼠Emd qPCR引物对，主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。 本引物为预先设计、经过qPCR验证、预混的引物对。

EMD Biosciences Alt Name pET28a Plasmid Type Bacterial Expression Expression Level High Cloning Method Unknown Size 5369 5' Sequencing 1 Primer T7 Fwd 5' Sequencing 1 Primer Sequence 5'd[TAATACGACTCACTATAGGG]3' Tag 1 His (Nterm and Cterm) Bacterial Resistance Kanamycin Notes Nterm thrombin cleavage site; a,b,c vary by …

The primer mix has been tested to generate satisfactory qPCR data on ABI 7900HT by using the following PCR program: Stage 1: Activation: 50 °C for 2 min; Stage 2: pre-soak:95 °C for 10 min; Stage 3: Denaturation: 95 °C for 15 sec, Annealing: 60°C for 1 min; Stage 4: Melting curve: 95°C for 15 sec, 60°C for 15 sec, 95°C for 15 sec.

Human EMD qPCR Primer Pair，即人EMD qPCR引物对，主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。 本引物为预先设计、经过qPCR验证、预混的引物对。

Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis. List Price: $169.00

qPCR引物描述 : Verified forward and reverse primers for analyzing the quantitative expression of gene . 質控信息: The primer mix has been verified to generate satisfactory qPCR data on Roche LightCycler480

Use Bio-Rad's PrimePCR assays, controls, templates for your target gene. Every primer pair is optimized, experimentally validated, and performance guaranteed.

Human EMD qPCR Primer Pair，即人EMD qPCR引物对，主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。 本引物为预先设计、经过qPCR验证、预混的引物对。

Position: position of primer (numbering according to the coding DNA Reference Sequence). Length (in bp): length of PCR-product in basepairs. Forward / reverse primer: sequence of forward and reverse primer.

A ready-to-use mix for SYBR Green qPCR, containing ROX reference and suitable for all qPCR instruments without adjusting the concentration of ROX. 1 vial of lyophilized qSTAR qPCR primer mix (1 nmol each primer, sufficient for 200 reactions). Before use, reconstitute the primer mix in 200 µl dH2O to make a final concentration of 10 µM.