Thermo Scientific Smart EPU Software is an innovative, easy-to-use data acquisition solution for single particle analysis (SPA) that focuses on automation and user guidance to increase throughput and deliver reproducible results.

The acronym EPU is from the Latin "E Pluribus Unum" — Out of Many, One. This reflects the actual pathway: a single 3D particle structure is extracted from numerous 2D images with multiple particles per image. EPU automates the most time-consuming step in the SPA workflow: the acquisition of useful images.

Smart EPU Software frees the operator from tedious and repetitive tasks while enabling new users to adopt the cryo-EM workflow faster. By encoding expert knowledge into neural networks, the Smart EPU software can automatically recognize and categorize areas in cryo-EM grids suitable for imaging, discarding those

2022年6月29日 · Made possible through forward-looking insights, feedback and close collaboration with structural biologists around the world, we introduce our new Thermo Scientific Smart EPU Software—a single particle analysis software solution that offers more automation capabilities for cryo-EM data acquisition than ever before.

Micro electron diffraction detector for organic molecules, proteins, and bio pharmaceuticals. The Thermo Scientific Ceta-D Camera is a scintillator-based camera optimized for low-dose diffraction data collection. It is ideal for working with dose-sensitive materials, such as proteins, pharmaceutical molecules, and organic materials.

为解决 CryoEM 成本高、设备受限问题，美国明尼苏达大学荷美尔研究所研究人员开展基于 EPU 软件的单颗粒 CryoEM 数据收集策略研究。 结果表明特定参数组合可提效，该成果对优化数据收集意义重大，强烈推荐科研读者阅读。

TEM delivers higher throughput and makes cryo-EM more accessible. In addition to the optional integrated Thermo Scientific ™ Falcon 4i Direct Electron Detector, and a low-energy-spread cold field emission gun (E-CFEG), it features Thermo Scientific EPU …

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2023年10月25日 · This quick guide provides key minimal steps for preparing the Microscope/EPU for single-particle data collection Please note that this guide might be slightly different for each...

Here, we show how the Thermo ScientificTM Glacios 2TM cryo transmission electron microscope (Cryo-TEM) with low-energy-spread Cold Field Emission Gun (E-CFEG) and combined with Thermo ScientificTM Smart EPU Software enables users of all expertise levels to acquire high-quality cryo-EM data.