Providing a biological scaffold (fibrin, poly(e)-caprolactone) to support ES cell transplantation and cellular regrowth—a particular hurdle in SCI repair—further enhanced functional recovery from …

2005年12月12日 · Since ES cells contribute to all tissues upon injection into a recipient blastocyst, including the germ line [3], [4] modification in an ES cell genome can be …

2005年12月12日 · An alternate approach is to establish ES cell lines from somatic cells through “therapeutic cloning” or somatic cell nuclear transfer [80], [81]. This technique refers to the …

2014年1月1日 · The efficiency in mouse ES cell derivation is influenced by genetic background. For example, ES cells can be easily derived from the inbred 129/ter-Sv strain, but less …

In addition, pre-conditioning of ES cells has been shown to promote survival of transplanted cells and provides a simple yet effective way of optimizing cell therapy (12, 13). However, the use of …

2017年5月9日 · ESCs cultured in 2i/LIF exhibit unique features including the elevated expression of pluripotent genes, the suppression of genes mediating cell differentiation, and global DNA …

2011年2月4日 · The ES cell lines were obtained from the Human Embryonic Stem Cell Facility of the Harvard Stem Cell Institute (17 ES cell lines) and from the WiCell Research Institute's …

2013年11月1日 · Hence, ES cell homeostasis ultimately depends on E-cad mediated cell–cell contacts and adhesion, which are required for communication and signaling. In contrast, …

2003年5月30日 · However, Stat3 is not ES cell-specific but is found in a variety of other cell types, often associated with differentiation (Hirano et al., 2000). Oct4 is restricted to pluripotent and …

2003年1月1日 · ES cell differentiation to the cardiac lineage facilitates developmental analyses of heart cells. In addition, these techniques can be employed in vitro: (1) to test the effects of …