2015年12月1日 · We present a new way of performing low energy transmission electron microscopy in an aberration corrected LEEM/PEEM instrument. We show a proof of principle where we measure transmitted electrons through a suspended graphene monolayer with a preliminary setup.

2021年4月1日 · TEM techniques for EV imaging are summarized and compared with respect to their applications, benefits and drawbacks. TEM contribution to the study of physical-chemical features of EVs is highlighted. Artifacts commonly encountered in the literature, technical challenges and misinterpretations are discussed.

这种新技术称为“ev-tem”，这是电子显微镜的一种新变体，它能将电子束对准样品，以便对其成像。 低能量电子 根据量子力学定律，电子也是波，就像可见光一样，但是波长要短得多。

2017年8月22日 · In cryo-TEM imaging, samples are directly applied onto an EM grid, vitrified and visualized, thus allowing for characterization of EVs near its native state. In this chapter, we describe a step-by-step guide for preparing EVs on the grid before TEM and cryo-TEM imaging.

We are developing a transmission electron microscope that operates at extremely low electron energies, 0-40 eV. We call this technique eV-TEM. Its feasibility is based on the fact that at very low electron energies the number of energy loss pathways decreases.

2020年9月21日 · The long and short axes of the DNA origami rectangles are discernable even after an hour of illumination with low energy electrons. In combination with recent developments in 2D membranes, allowing for versatile sample preparation, eV-TEM is paving the way to damage-free imaging of biological samples at nm resolution.

2015年6月26日 · We are developing a transmission electron microscope that operates at extremely low electron energies, 0-40eV. We call this technique eV-TEM. Its feasibility is based on the fact that at very low...

2019年1月9日 · Transmission electron microscopy (TEM) has nanometre resolution and can be used to distinguish single extracellular vesicles (EVs) from non-EV particles. TEM images of EVs are a result of operator image selection.

In this chapter, we describe a step-by-step guide for preparing EVs on the grid before TEM and cryo-TEM imaging. Finally, we provide a guide to an automated image-processing analysis to provide the size distribution of EVs.

2018年12月24日 · Transmission electron microscopy (TEM) is the most common type of electron microscopies for EV imaging, such as exosomes , microvesicles , oncosomes and migrasomes . The samples prepared for TEM imaging are first fixed and later dehydrated.